BACKGROUND: The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC). METHODS AND RESULTS: Comparing with adjacent tissues (n=75), miR-30c has a lower expression in lung cancer specimens (n=75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3'-untranslated regions (3'-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c. CONCLUSION: miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC.
BACKGROUND: The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC). METHODS AND RESULTS: Comparing with adjacent tissues (n=75), miR-30c has a lower expression in lung cancer specimens (n=75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3'-untranslated regions (3'-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c. CONCLUSION:miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC.
Authors: Lauren MacDonagh; Michael F Gallagher; Brendan Ffrench; Claudia Gasch; Steven G Gray; Marie Reidy; Siobhan Nicholson; Niamh Leonard; Ronan Ryan; Vincent Young; John J O'Leary; Sinead Cuffe; Stephen P Finn; Kenneth J O'Byrne; Martin P Barr Journal: Transl Lung Cancer Res Date: 2021-04