| Literature DB >> 23978265 |
Thua N N Tran1, Jinhui Cui, Mark R Hartman, Songming Peng, Hisakage Funabashi, Faping Duan, Dayong Yang, John C March, John T Lis, Haixin Cui, Dan Luo.
Abstract
Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23978265 PMCID： PMC4237005 DOI： 10.1021/ja405872g
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419