BACKGROUND: A sensitive and reliable rapid diagnostic test (RDT) which should have comparable diagnostic performance against reference host serological methods is urgently needed for use in point-of-care (POC) diagnosis of intestinal schistosomiasis in pre school-aged children. METHODS: The diagnostic accuracy of a RDT incorporating Schistosoma mansoni cercarial transformation fluid (SmCTF) for anti-schistosome antibody detection was evaluated with serum samples from a cohort of children from Uganda: 42 children aged under the age of 3 years and 40 children aged between 3 and 5 years. The infection status of these children had been previously determined by inspection of quadruplicate Kato-Katz faecal smears, a single urine circulating cathodic antigen (CCA) dipstick and antibody titres to S. mansoni soluble egg antigen (SmSEA) with a commercially available ELISA. RESULTS: Upon comparison with quadruplicate Kato-Katz the sensitivity and specificity of the RDT were 75.7% and 31.1%, respectively. When using the SmSEA-ELISA as an alternate reference test, the RDT achieved 81.3% sensitivity and 61.1% specificity. Sensitivity and specificity compared to the urine-CCA test was 74.5% and 32.3% respectively. Sensitivity differed significantly according to age group. CONCLUSIONS: The performance of the RDT within this study appeared favourable when compared with the currently-available SmSEA-ELISA. Looking to the future a serological POC test would be particularly promising for use in disease mapping in younger children especially in guiding administration of praziquantel treatment in selective treatment settings.
BACKGROUND: A sensitive and reliable rapid diagnostic test (RDT) which should have comparable diagnostic performance against reference host serological methods is urgently needed for use in point-of-care (POC) diagnosis of intestinal schistosomiasis in pre school-aged children. METHODS: The diagnostic accuracy of a RDT incorporating Schistosoma mansoni cercarial transformation fluid (SmCTF) for anti-schistosome antibody detection was evaluated with serum samples from a cohort of children from Uganda: 42 children aged under the age of 3 years and 40 children aged between 3 and 5 years. The infection status of these children had been previously determined by inspection of quadruplicate Kato-Katz faecal smears, a single urine circulating cathodic antigen (CCA) dipstick and antibody titres to S. mansoni soluble egg antigen (SmSEA) with a commercially available ELISA. RESULTS: Upon comparison with quadruplicate Kato-Katz the sensitivity and specificity of the RDT were 75.7% and 31.1%, respectively. When using the SmSEA-ELISA as an alternate reference test, the RDT achieved 81.3% sensitivity and 61.1% specificity. Sensitivity and specificity compared to the urine-CCA test was 74.5% and 32.3% respectively. Sensitivity differed significantly according to age group. CONCLUSIONS: The performance of the RDT within this study appeared favourable when compared with the currently-available SmSEA-ELISA. Looking to the future a serological POC test would be particularly promising for use in disease mapping in younger children especially in guiding administration of praziquantel treatment in selective treatment settings.
Authors: Paul L A M Corstjens; Claudia J De Dood; Dieuwke Kornelis; Elisa M Tjon Kon Fat; R Alan Wilson; Thomas M Kariuki; Ruth K Nyakundi; Philip T Loverde; William R Abrams; Hans J Tanke; Lisette Van Lieshout; André M Deelder; Govert J Van Dam Journal: Parasitology Date: 2014-06-16 Impact factor: 3.234
Authors: Alda Maria Soares Silveira; Emanuele Gama Dutra Costa; Debalina Ray; Brian M Suzuki; Michael H Hsieh; Lucia Alves de Oliveira Fraga; Conor R Caffrey Journal: PLoS Negl Trop Dis Date: 2016-01-11
Authors: Nupur Kittur; Jennifer D Castleman; Carl H Campbell; Charles H King; Daniel G Colley Journal: Am J Trop Med Hyg Date: 2016-01-11 Impact factor: 2.345
Authors: Stephen A Spencer; James M St John Penney; Hannah J Russell; Anthony P Howe; Cortland Linder; Andriamahitsisambatra L D Rakotomampianina; Anjara M Nandimbiniaina; S Bertel Squire; J Russell Stothard; Amaya L Bustinduy; Alain M Rahetilahy Journal: Parasit Vectors Date: 2017-06-24 Impact factor: 3.876
Authors: Oscar A Nyangiri; Sokouri A Edwige; Mathurin Koffi; Estelle Mewamba; Gustave Simo; Joyce Namulondo; Julius Mulindwa; Jacent Nassuuna; Alison Elliott; Kévin Karume; Dieudonne Mumba; P L A M Corstjens; M Casacuberta-Partal; G J van Dam; Bruno Bucheton; Harry Noyes; Enock Matovu Journal: AAS Open Res Date: 2021-12-15
Authors: Anthony Danso-Appiah; Jonathan Minton; Daniel Boamah; Joseph Otchere; Richard H Asmah; Mark Rodgers; Kwabena M Bosompem; Paolo Eusebi; Sake J De Vlas Journal: Bull World Health Organ Date: 2016-04-22 Impact factor: 9.408