| Literature DB >> 23960848 |
A S Tripathi1, I Sheikh, A P Dewani, P G Shelke, R L Bakal, A V Chandewar, P M Mazumder.
Abstract
Sildenafil citrate (SIL) is used in the treatment of erectile dysfunction and other chronic disorders. For the pharmacokinetic investigation of SIL we developed a simple and sensitive method for the estimation of SIL in rat plasma by reverse phase high-performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 μl of acetonitrile and 5 ml of diethyl ether. Chromatographic separation was achieved on C18 column using methanol:water (85:15 v/v) as mobile phase at a flow rate of 1 ml/min and UV detection at 230 nm. The retention time of SIL was found to be 4.0 min having a separation time less than 5 min. The developed method was validated for accuracy, precision, linearity and recovery. Linearity studies were found to be acceptable over the range of 0.1-6 μg/ml. The method was successfully applied for the analysis of rat plasma sample for the application in pharmacokinetic study, drug interaction, bioavailability and bioequivalence.Entities:
Keywords: % RSD, % relative standard deviation; DAD, diode-array detection; RP-HPLC; Rat plasma; SIL, sildenafil citrate; Sildenafil citrate
Year: 2012 PMID： 23960848 PMCID： PMC3745091 DOI： 10.1016/j.jsps.2012.09.003
Source DB: PubMed Journal: Saudi Pharm J ISSN： 1319-0164 Impact factor: 4.330