Literature DB >> 23960025

Bioorthogonal approach to identify unsuspected drug targets in live cells.

Katherine S Yang1, Ghyslain Budin, Carlos Tassa, Olivier Kister, Ralph Weissleder.   

Abstract

A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  bioorthogonal reaction; cleavable linkers; drug targets; proteins; proteomics

Mesh：

Year:  2013        PMID： 23960025      PMCID： PMC3856564          DOI： 10.1002/anie.201304096

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


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