INTRODUCTION: The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections. METHODS: Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted. RESULTS: Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05). CONCLUSIONS: The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.
INTRODUCTION: The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections. METHODS: Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted. RESULTS: Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05). CONCLUSIONS: The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.
Authors: Marlos Barbosa-Ribeiro; Rodrigo Arruda-Vasconcelos; Lidiane M Louzada; Danielle G Dos Santos; Fernando D Andreote; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2020-08-28 Impact factor: 3.573
Authors: Isabela N Rôças; Flávio R F Alves; Caio T C C Rachid; Kenio C Lima; Isauremi V Assunção; Patrícia N Gomes; José F Siqueira Journal: PLoS One Date: 2016-05-02 Impact factor: 3.240
Authors: José F Siqueira; Henrique S Antunes; Isabela N Rôças; Caio T C C Rachid; Flávio R F Alves Journal: PLoS One Date: 2016-09-30 Impact factor: 3.240