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Literature DB >> 2394445

DNA sequence analysis of the KM19 locus linked to cystic fibrosis. Design of new oligonucleotides to remove non-specific PCR products.

R Anwar¹, K Murray, P J Hedge, J C Smith, A F Markham.

Abstract

The PstI polymorphism detected by probe KM19 is a highly informative marker in linkage disequilibrium with the cystic fibrosis locus and has been used extensively for prenatal diagnosis. The currently available primers used for polymerase chain reaction- (PCR-) based analysis of this locus have been shown to produce spurious amplification products. In this report, we describe the sequence of the KM19 locus and the major contaminating PCR product. We have used this information to design a more specific amplification procedure for analysis of the KM19 locus.

Entities: Chemical Disease Gene

Mesh：

Substances：
DNA Probes

Year: 1990 PMID： 2394445 DOI： 10.1007/bf00206754

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

16 in total

1. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

Authors: T Triglia; M G Peterson; D J Kemp
Journal: Nucleic Acids Res Date: 1988-08-25 Impact factor: 16.971

2. Experience with new DNA markers for the diagnosis of cystic fibrosis.

Authors: A L Beaudet; J E Spence; M Montes; W E O'Brien; X Estivill; M Farrall; R Williamson
Journal: N Engl J Med Date: 1988-01-07 Impact factor: 91.245

3. Sequence analysis of the human major histocompatibility gene SX alpha.

Authors: J M Boss; R Mengler; K Okada; C Auffray; J L Strominger
Journal: Mol Cell Biol Date: 1985-10 Impact factor: 4.272

4. Patterns of polymorphism and linkage disequilibrium for cystic fibrosis.

Authors: X Estivill; P J Scambler; B J Wainwright; K Hawley; P Frederick; M Schwartz; M Baiget; J Kere; R Williamson; M Farrall
Journal: Genomics Date: 1987-11 Impact factor: 5.736

5. Genetic linkage map of human chromosome 7 with 63 DNA markers.

Authors: D Barker; P Green; R Knowlton; J Schumm; E Lander; A Oliphant; H Willard; G Akots; V Brown; T Gravius
Journal: Proc Natl Acad Sci U S A Date: 1987-11 Impact factor: 11.205

6. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.

Authors: J R Riordan; J M Rommens; B Kerem; N Alon; R Rozmahel; Z Grzelczak; J Zielenski; S Lok; N Plavsic; J L Chou
Journal: Science Date: 1989-09-08 Impact factor: 47.728

7. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.

Authors: C R Newton; N Kalsheker; A Graham; S Powell; A Gammack; J Riley; A F Markham
Journal: Nucleic Acids Res Date: 1988-09-12 Impact factor: 16.971

DNA sequence analysis of the KM19 locus linked to cystic fibrosis. Design of new oligonucleotides to remove non-specific PCR products.

1. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

2. Experience with new DNA markers for the diagnosis of cystic fibrosis.

3. Sequence analysis of the human major histocompatibility gene SX alpha.

4. Patterns of polymorphism and linkage disequilibrium for cystic fibrosis.

5. Genetic linkage map of human chromosome 7 with 63 DNA markers.

6. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.

7. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.

8. Identification of the cystic fibrosis gene: chromosome walking and jumping.

9. Cystic fibrosis locus defined by a genetically linked polymorphic DNA marker.

10. Localization of cystic fibrosis locus to human chromosome 7cen-q22.

1. Cystic fibrosis in the Basque country: high frequency of mutation delta F508 in patients of Basque origin.

2. Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families.