Literature DB >> 2393575

Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction.

N Kusukawa1, T Uemori, K Asada, I Kato.   

Abstract

A simple and reliable method is described for direct sequencing of material generated by the polymerase chain reaction. The protocol is based on the purification of the amplified double-stranded product by polyethylene glycol precipitation, annealing of primer with template by a "snap-cooling" procedure and sequencing by the dideoxy chain termination method with the use of Klenow fragment or Taq polymerase. The limit of the size of PCR products that can be sequenced is also discussed.

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Year:  1990        PMID： 2393575

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  50 in total

1.  Acholeplasma laidlawii has tRNA genes in the 16S-23S spacer of the rRNA operon.

Authors:  T Nakagawa; T Uemori; K Asada; I Kato; R Harasawa
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

2.  Identical genes confer high-level resistance to gentamicin upon Enterococcus faecalis, Enterococcus faecium, and Streptococcus agalactiae.

Authors:  A Kaufhold; A Podbielski; T Horaud; P Ferrieri
Journal:  Antimicrob Agents Chemother       Date:  1992-06       Impact factor: 5.191

3.  Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.

Authors:  C L Yang; L S Chang; P Zhang; H Hao; L Zhu; N L Toomey; M Y Lee
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

4.  Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.

Authors:  B J Mann; B E Torian; T S Vedvick; W A Petri
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

5.  High fidelity developmental excision of Tec1 transposons and internal eliminated sequences in Euplotes crassus.

Authors:  S L Tausta; L R Turner; L K Buckley; L A Klobutcher
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

6.  Characterization of large deletions occurring during a single round of retrovirus vector replication: novel deletion mechanism involving errors in strand transfer.

Authors:  G A Pulsinelli; H M Temin
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

7.  A simple method for cloning blunt ended DNA fragments.

Authors:  G J Bhat; M J Lodes; P J Myler; K D Stuart
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

8.  Sequence-tagged-site-facilitated PCR for barley genome mapping.

Authors:  S Tragoonrung; V Kanazin; P M Hayes; T K Blake
Journal:  Theor Appl Genet       Date:  1992-09       Impact factor: 5.699

9.  Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH.

Authors:  H Yigit; W S Reznikoff
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

10.  Helicobacter pylori isolated from the domestic cat: public health implications.

Authors:  L K Handt; J G Fox; F E Dewhirst; G J Fraser; B J Paster; L L Yan; H Rozmiarek; R Rufo; I H Stalis
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441
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