Haidy E Zidan1, Noha A Rezk, Doaa Mohammed. 1. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: haidy_menna@hotmail.com.
Abstract
OBJECTIVE: To investigate the association of combined MTHFR C677T and A1298C gene polymorphisms with congenital heart diseases (CHD) in Egyptian children and their mothers and to determine their effect on homocysteine level in these children. MATERIAL AND METHODS: MTHFR C677T and A1298C polymorphisms were genotyped in 160 Egyptian children (80 patients with CHD and 80 healthy controls) and their mothers using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), while, homocysteine (Hcy) level was measured optically by enzymatic method. RESULTS: We found that MTHFR 677TT genotype, T allele, 1298CC genotype, and C allele were associated with 2.61, 2.0, 2.91 and 1.99 fold increased risk of CHD in Egyptian children respectively. Furthermore, the frequencies of MTHFR 1298AC and CC genotypes and C allele significantly increased in mothers with CHD affected children. The homocysteine levels were significantly increased in MTHFR 677TT and 1298CC genotypes in children with CHD. CONCLUSIONS: Our study demonstrated an association of MTHFR A1298C polymorphisms with CHD in Egyptian children and their mothers, while, MTHFR C677T polymorphisms were significantly associated with the risk of CHD in the children only. An association between combined MTHFR A1298C and C677T polymorphisms and CHD was recorded in the children and their mothers. Also, homocysteine levels were significantly increased with both MTHFR 677TT and 1298CC genotypes in Egyptian children with CHD.
OBJECTIVE: To investigate the association of combined MTHFRC677T and A1298C gene polymorphisms with congenital heart diseases (CHD) in Egyptian children and their mothers and to determine their effect on homocysteine level in these children. MATERIAL AND METHODS:MTHFRC677T and A1298C polymorphisms were genotyped in 160 Egyptian children (80 patients with CHD and 80 healthy controls) and their mothers using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), while, homocysteine (Hcy) level was measured optically by enzymatic method. RESULTS: We found that MTHFR 677TT genotype, T allele, 1298CC genotype, and C allele were associated with 2.61, 2.0, 2.91 and 1.99 fold increased risk of CHD in Egyptian children respectively. Furthermore, the frequencies of MTHFR 1298AC and CC genotypes and C allele significantly increased in mothers with CHD affected children. The homocysteine levels were significantly increased in MTHFR 677TT and 1298CC genotypes in children with CHD. CONCLUSIONS: Our study demonstrated an association of MTHFRA1298C polymorphisms with CHD in Egyptian children and their mothers, while, MTHFRC677T polymorphisms were significantly associated with the risk of CHD in the children only. An association between combined MTHFRA1298C and C677T polymorphisms and CHD was recorded in the children and their mothers. Also, homocysteine levels were significantly increased with both MTHFR 677TT and 1298CC genotypes in Egyptian children with CHD.
Authors: Nicholas Ekow Thomford; Kevin Dzobo; Nana Akyaa Yao; Emile Chimusa; Jonathan Evans; Emmanuel Okai; Paul Kruszka; Maximilian Muenke; Gordon Awandare; Ambroise Wonkam; Collet Dandara Journal: OMICS Date: 2018-05
Authors: Hany M M Abdel Allah; Walid E Zahran; Samir A El-Masry; Mahmoud El-Bendary; Ahmed F Soliman Journal: Clin Exp Med Date: 2021-07-23 Impact factor: 3.984