Literature DB >> 23912761

Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels.

Yang Pu1, Jianpeng Xue, Wubao Wang, Baogang Xu, Yueqing Gu, Rui Tang, Ellen Ackerstaff, Jason A Koutcher, Samuel Achilefu, Robert R Alfano.   

Abstract

The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

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Year:  2013        PMID： 23912761      PMCID： PMC3763931          DOI： 10.1117/1.JBO.18.8.087002

Source DB:  PubMed          Journal:  J Biomed Opt        ISSN： 1083-3668            Impact factor:   3.170


  12 in total

1.  Near infrared photonic finger imager for prostate cancer screening.

Authors:  Y Pu; W B Wang; M Xu; G C Tang; Y Budansky; M Sharanov; S Achilefu; J A Eastham; R R Alfano
Journal:  Technol Cancer Res Treat       Date:  2011-12

2.  Modulation of nuclear internalization of Tat peptides by fluorescent dyes and receptor-avid peptides.

Authors:  Duanwen Shen; Kexian Liang; Yunpeng Ye; Elizabeth Tetteh; Samuel Achilefu
Journal:  FEBS Lett       Date:  2007-04-04       Impact factor: 4.124

3.  Time-resolved transillumination for medical diagnostics.

Authors:  S Andersson-Engels; R Berg; S Svanberg; O Jarlman
Journal:  Opt Lett       Date:  1990-11-01       Impact factor: 3.776

4.  Native fluorescence spectra of human cancerous and normal breast tissues analyzed with non-negative constraint methods.

Authors:  Yang Pu; Wubao Wang; Yuanlong Yang; Robert R Alfano
Journal:  Appl Opt       Date:  2013-02-20       Impact factor: 1.980

5.  Characterization and three-dimensional localization of cancerous prostate tissue using backscattering scanning polarization imaging and independent component analysis.

Authors:  Yang Pu; Wubao Wang; Min Xu; James A Eastham; Guicheng Tang; Robert R Alfano
Journal:  J Biomed Opt       Date:  2012-08       Impact factor: 3.170

6.  Light sheds light on cancer--distinguishing malignant tumors from benign tissues and tumors.

Authors:  R R Alfano; B B Das; J Cleary; R Prudente; E J Celmer
Journal:  Bull N Y Acad Med       Date:  1991 Mar-Apr

7.  Native fluorescence spectroscopic evaluation of chemotherapeutic effects on malignant cells using nonnegative matrix factorization analysis.

Authors:  Y Pu; G C Tang; W B Wang; H E Savage; S P Schantz; R R Alfano
Journal:  Technol Cancer Res Treat       Date:  2011-04

8.  Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength.

Authors:  Yang Pu; Wubao Wang; Wubao B Wang; Guichen Tang; Guichen C Tang; Robert R Alfano
Journal:  J Biomed Opt       Date:  2010 Jul-Aug       Impact factor: 3.170

Review 9.  Cell lines used in prostate cancer research: a compendium of old and new lines--part 1.

Authors:  R E Sobel; M D Sadar
Journal:  J Urol       Date:  2005-02       Impact factor: 7.450

10.  Stokes shift spectroscopy highlights differences of cancerous and normal human tissues.

Authors:  Yang Pu; Wuabo Wang; Yuanlong Yang; R R Alfano
Journal:  Opt Lett       Date:  2012-08-15       Impact factor: 3.776
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  4 in total

1.  Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure.

Authors:  João Lagarto; Benjamin T Dyer; Clifford Talbot; Markus B Sikkel; Nicholas S Peters; Paul M W French; Alexander R Lyon; Chris Dunsby
Journal:  Biomed Opt Express       Date:  2015-01-07       Impact factor: 3.732

2.  Optical Signatures Derived From Deep UV to NIR Excitation Discriminates Healthy Samples From Low and High Grades Glioma.

Authors:  Hussein Mehidine; Audrey Chalumeau; Fanny Poulon; Frédéric Jamme; Pascale Varlet; Bertrand Devaux; Matthieu Refregiers; Darine Abi Haidar
Journal:  Sci Rep       Date:  2019-06-19       Impact factor: 4.379

3.  Statistically strong label-free quantitative identification of native fluorophores in a biological sample.

Authors:  Saabah B Mahbub; Martin Plöschner; Martin E Gosnell; Ayad G Anwer; Ewa M Goldys
Journal:  Sci Rep       Date:  2017-11-17       Impact factor: 4.379

4.  Isobaric Tag-Based Protein Profiling across Eight Human Cell Lines Using High-Field Asymmetric Ion Mobility Spectrometry and Real-Time Database Searching.

Authors:  Xinyue Liu; Steven P Gygi; Joao A Paulo
Journal:  Proteomics       Date:  2020-10-26       Impact factor: 3.984
  4 in total

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