BACKGROUND: As the worldwide breast cancer burden increases, non-invasive tools, such as ultrasonography and thermography are being increasingly sought after. N-methyl-N-nitrosourea-induced rat mammary tumors are important tools to investigate the usefulness of such imaging techniques. OBJECTIVE: This study aimed to integrate both ultrasonographic and thermographic approaches to the vascularization and the superficial temperature of chemically-induced rat mammary tumors. MATERIALS AND METHODS: Twenty-five female Sprague-Dawley rats were divided into two groups: group I (intraperitoneally administered with N-methyl-N-nitrosourea) and group II (control group). Thirty-five weeks after the administration of the carcinogen, mammary tumors were evaluated using Power Doppler, B Flow and Contrast-enhanced ultrasound, thermography and histology analyses. RESULTS: Group I animals showed an average of 2.5 mammary tumors per animal, mostly papillary and cribriform non-invasive carcinomas. B Flow detected higher counts of colour pixels than Power Doppler. Contrast-enhanced ultrasound analysis showed a centripetal enhancement order of contrast agent and clear margins. Maximum tumor temperature and thermal amplitude determined by thermography were significantly correlated with tumor volume and with color pixel density, determined by Power Doppler. CONCLUSION: B Flow was more sensitive than Power Doppler in detecting tumor vessels, but Power Doppler correlates with thermographic data concerning superficial temperature and may reflect tumor angiogenesis.
BACKGROUND: As the worldwide breast cancer burden increases, non-invasive tools, such as ultrasonography and thermography are being increasingly sought after. N-methyl-N-nitrosourea-induced rat mammary tumors are important tools to investigate the usefulness of such imaging techniques. OBJECTIVE: This study aimed to integrate both ultrasonographic and thermographic approaches to the vascularization and the superficial temperature of chemically-induced rat mammary tumors. MATERIALS AND METHODS: Twenty-five female Sprague-Dawley rats were divided into two groups: group I (intraperitoneally administered with N-methyl-N-nitrosourea) and group II (control group). Thirty-five weeks after the administration of the carcinogen, mammary tumors were evaluated using Power Doppler, B Flow and Contrast-enhanced ultrasound, thermography and histology analyses. RESULTS: Group I animals showed an average of 2.5 mammary tumors per animal, mostly papillary and cribriform non-invasive carcinomas. B Flow detected higher counts of colour pixels than Power Doppler. Contrast-enhanced ultrasound analysis showed a centripetal enhancement order of contrast agent and clear margins. Maximum tumor temperature and thermal amplitude determined by thermography were significantly correlated with tumor volume and with color pixel density, determined by Power Doppler. CONCLUSION: B Flow was more sensitive than Power Doppler in detecting tumor vessels, but Power Doppler correlates with thermographic data concerning superficial temperature and may reflect tumor angiogenesis.
Authors: Yewei Liu; Ting Yin; Yuanbo Feng; Marlein Miranda Cona; Gang Huang; Jianjun Liu; Shaoli Song; Yansheng Jiang; Qian Xia; Johannes V Swinnen; Guy Bormans; Uwe Himmelreich; Raymond Oyen; Yicheng Ni Journal: Quant Imaging Med Surg Date: 2015-10
Authors: Iwona Malicka; Katarzyna Siewierska; Bartosz Pula; Christopher Kobierzycki; Dominik Haus; Urszula Paslawska; Marek Cegielski; Piotr Dziegiel; Marzena Podhorska-Okolow; Marek Wozniewski Journal: Exp Biol Med (Maywood) Date: 2015-05-19
Authors: Antonieta Alvarado; Rui M Gil da Costa; Ana I Faustino-Rocha; Rita Ferreira; Carlos Lopes; Paula A Oliveira; Bruno Colaço Journal: Int J Exp Pathol Date: 2017-02 Impact factor: 1.925
Authors: Ana I Faustino-Rocha; Adelina Gama; Paula A Oliveira; Antonieta Alvarado; Maria J Neuparth; Rita Ferreira; Mário Ginja Journal: Clin Exp Med Date: 2016-04-19 Impact factor: 3.984
Authors: Amanda Mara Teles; Leticia Prince Pereira Pontes; Sulayne Janayna Araújo Guimarães; Ana Luiza Butarelli; Gabriel Xavier Silva; Flavia Raquel Fernandes do Nascimento; Geusa Felipa de Barros Bezerra; Carla Junqueira Moragas-Tellis; Rui Miguel Gil da Costa; Marcos Antonio Custódio Neto da Silva; Fernando Almeida-Souza; Kátia da Silva Calabrese; Ana Paula Silva Azevedo-Santos; Maria do Desterro Soares Brandão Nascimento Journal: Mar Drugs Date: 2020-10-29 Impact factor: 5.118