| Literature DB >> 23906611 |
S Vimal1, S Abdul Majeed, G Taju, K S N Nambi, N Sundar Raj, N Madan, M A Farook, T Rajkumar, D Gopinath, A S Sahul Hameed.
Abstract
The present study examines the use of CS/TPP nanoparticles for gene delivery in different tissues of shrimp through oral route. The viral gene of WSSV was used to construct DNA vaccines using pcDNA 3.1, a eukaryotic expression vector and the constructs were named as pVP28. The CS/TPP nanoparticles were synthesized by ionic gelation process and these particles were characterized. The structure and morphology of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT assay using fish cell line. The expression of gene was confirmed by Immuno-dot blot, ELISA and RT-PCR analyses. The results indicate that DNA can be easily delivered into shrimp by feeding with CS/TPP nanoparticles.Entities:
Keywords: CS/TPP nanoparticles; Immuno-dot blot; In vivo gene expression; Real-time PCR; pVP28 DNA
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Year: 2013 PMID： 23906611 DOI： 10.1016/j.actatropica.2013.07.013
Source DB: PubMed Journal: Acta Trop ISSN： 0001-706X Impact factor: 3.112