Onakpa Michael Monday1, Asuzu Isaac Uzoma. 1. Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Abuja, PMB 117, Abuja, Nigeria. onakpamm@yahoo.com
Abstract
OBJECTIVE: To investigate the antidiabetic, hypolipidaemic activities and histopathological changes of Icacina trichantha (I. trichantha) tuber extract in alloxan induced diabetic rats. METHODS: In the present study, 80% methanol extract of I. trichantha tuber was tested on alloxan induced diabetic rats. They were randomly grouped into control (distilled water and glibenclamide) and experimental (200, 400 and 600 mg/kg body weight). Diabetes was induced by a single intraperitoneal injection of 160 mg/kg body weight of alloxan. Blood glucose levels were measured using blood glucose test strips with AccuCheck Advantage II glucometer at 1, 3, 6, and 24 h on the first day and 1 h after treatment on Day 7, 14 and 21. Blood samples were collected and centrifuged to separate serum for estimation of lipid profile and other biochemical parameters. Histopathological changes in diabetic rats pancreas were also studied after extract treatment. RESULTS: Daily oral administration of I. trichantha tuber extract (200, 400, and 600 mg/kg body weight) and glibenclamide (2 mg/kg) showed beneficial effects on blood glucose level (P<0.01) as well as improving liver, kidney functions and hyperlipidaemia due to diabetes. The extract had a favourable effect on the histopathological changes of the pancreas in alloxan induced diabetes. CONCLUSIONS: I. trichantha tuber extracts posses antidiabetic activities as well as improve liver and renal profile and total lipids levels. I. trichantha tuber extracts also have favourable effects to inhibit the histopathological changes of the pancreas in alloxan induced diabetes.
OBJECTIVE: To investigate the antidiabetic, hypolipidaemic activities and histopathological changes of Icacina trichantha (I. trichantha) tuber extract in alloxan induced diabeticrats. METHODS: In the present study, 80% methanol extract of I. trichantha tuber was tested on alloxan induced diabeticrats. They were randomly grouped into control (distilled water and glibenclamide) and experimental (200, 400 and 600 mg/kg body weight). Diabetes was induced by a single intraperitoneal injection of 160 mg/kg body weight of alloxan. Blood glucose levels were measured using blood glucose test strips with AccuCheck Advantage II glucometer at 1, 3, 6, and 24 h on the first day and 1 h after treatment on Day 7, 14 and 21. Blood samples were collected and centrifuged to separate serum for estimation of lipid profile and other biochemical parameters. Histopathological changes in diabeticrats pancreas were also studied after extract treatment. RESULTS: Daily oral administration of I. trichantha tuber extract (200, 400, and 600 mg/kg body weight) and glibenclamide (2 mg/kg) showed beneficial effects on blood glucose level (P<0.01) as well as improving liver, kidney functions and hyperlipidaemia due to diabetes. The extract had a favourable effect on the histopathological changes of the pancreas in alloxan induced diabetes. CONCLUSIONS: I. trichantha tuber extracts posses antidiabetic activities as well as improve liver and renal profile and total lipids levels. I. trichantha tuber extracts also have favourable effects to inhibit the histopathological changes of the pancreas in alloxan induced diabetes.
Authors: Tim Strate; Oliver Mann; Helge Kleinhans; Skelkim Rusani; Claus Schneider; Emre Yekebas; Marc Freitag; Thomas Standl; Christian Bloechle; Jakob R Izbicki Journal: Pancreas Date: 2005-04 Impact factor: 3.327
Authors: Ming Zhao; Michael Monday Onakpa; Wei-Lun Chen; Bernard D Santarsiero; Steven M Swanson; Joanna E Burdette; Isaac Uzoma Asuzu; Chun-Tao Che Journal: J Nat Prod Date: 2015-03-17 Impact factor: 4.050
Authors: Brian Guo; Monday M Onakpa; Xiao-Jun Huang; Bernard D Santarsiero; Wei-Lun Chen; Ming Zhao; Xiao-Qi Zhang; Steven M Swanson; Joanna E Burdette; Chun-Tao Che Journal: J Nat Prod Date: 2016-06-24 Impact factor: 4.050