CONTEXT: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. OBJECTIVE: Identification of a characteristic circulating miRNAs profile in melanoma patients. METHODS: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. RESULTS: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. DISCUSSION AND CONCLUSIONS: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.
CONTEXT: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. OBJECTIVE: Identification of a characteristic circulating miRNAs profile in melanomapatients. METHODS: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanomapatients and healthy donors. RESULTS: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanomapatients from healthy donors, metastatic colon and renal cancerpatients. DISCUSSION AND CONCLUSIONS: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.
Authors: Nathaniel H Fleming; Judy Zhong; Inês Pires da Silva; Eleazar Vega-Saenz de Miera; Bobbi Brady; Sung Won Han; Doug Hanniford; Jinhua Wang; Richard L Shapiro; Eva Hernando; Iman Osman Journal: Cancer Date: 2014-08-25 Impact factor: 6.860
Authors: Mitchell S Stark; Kerenaftali Klein; Benjamin Weide; Lauren E Haydu; Annette Pflugfelder; Yue Hang Tang; Jane M Palmer; David C Whiteman; Richard A Scolyer; Graham J Mann; John F Thompson; Georgina V Long; Andrew P Barbour; H Peter Soyer; Claus Garbe; Adrian Herington; Pamela M Pollock; Nicholas K Hayward Journal: EBioMedicine Date: 2015-05-12 Impact factor: 8.143