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Literature DB >> 23893717

A suite of solid-state NMR experiments for RNA intranucleotide resonance assignment in a 21 kDa protein-RNA complex.

Alexander Marchanka¹, Bernd Simon, Teresa Carlomagno.

Abstract

Intranucleotide resonance of the 26mer box C/D RNA in complex with the L7Ae protein were assigned by solid-state NMR (ssNMR; see picture) spectroscopy. This investigation opens the way for studying RNA in large protein-RNA complexes by ssNMR spectroscopy.

Keywords: NMR spectroscopy; RNA; RNA-protein complexes; structure elucidation

Mesh：

Substances：
Proteins
RNA

Year: 2013 PMID： 23893717 DOI： 10.1002/anie.201304779

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

13 in total

1. Regio-selective chemical-enzymatic synthesis of pyrimidine nucleotides facilitates RNA structure and dynamics studies.

Authors: Luigi J Alvarado; Regan M LeBlanc; Andrew P Longhini; Sarah C Keane; Niyati Jain; Zehra F Yildiz; Blanton S Tolbert; Victoria M D'Souza; Michael F Summers; Christoph Kreutz; T Kwaku Dayie
Journal: Chembiochem Date: 2014-06-20 Impact factor: 3.164

2. Chemo-enzymatic synthesis of selectively ¹³C/¹⁵N-labeled RNA for NMR structural and dynamics studies.

Authors: Luigi J Alvarado; Andrew P Longhini; Regan M LeBlanc; Bin Chen; Christoph Kreutz; T Kwaku Dayie
Journal: Methods Enzymol Date: 2014 Impact factor: 1.600

Review 3. Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.

Authors: Caitlin M Quinn; Tatyana Polenova
Journal: Q Rev Biophys Date: 2017-01 Impact factor: 5.318

4. Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR.

Authors: Gili Abramov; Amir Goldbourt
Journal: J Biomol NMR Date: 2014-05-30 Impact factor: 2.835

A suite of solid-state NMR experiments for RNA intranucleotide resonance assignment in a 21 kDa protein-RNA complex.

1. Regio-selective chemical-enzymatic synthesis of pyrimidine nucleotides facilitates RNA structure and dynamics studies.

2. Chemo-enzymatic synthesis of selectively ¹³C/¹⁵N-labeled RNA for NMR structural and dynamics studies.

Review 3. Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.

4. Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR.

Review 5. Isotope labeling for studying RNA by solid-state NMR spectroscopy.

6. Gd³⁺-chelated lipid accelerates solid-state NMR spectroscopy of seven-transmembrane proteins.

7. Dynamic nuclear polarization of nucleic acid with endogenously bound manganese.

8. RNA structure determination by solid-state NMR spectroscopy.

9. Bacteriophage Tail-Tube Assembly Studied by Proton-Detected 4D Solid-State NMR.

10. Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS.