Literature DB >> 23893485

Structural integrity of centromeric chromatin and faithful chromosome segregation requires Pat1.

Prashant K Mishra1, Alicia R Ottmann, Munira A Basrai.   

Abstract

The kinetochore (centromeric DNA and associated protein complex) is essential for faithful chromosome segregation and maintenance of genome stability. Here we report that an evolutionarily conserved protein Pat1 is a structural component of Saccharomyces cerevisiae kinetochore and associates with centromeres in a NDC10-dependent manner. Consistent with a role for Pat1 in kinetochore structure and function, a deletion of PAT1 results in delay in sister chromatid separation, errors in chromosome segregation, and defects in structural integrity of centromeric chromatin. Pat1 is involved in topological regulation of minichromosomes as altered patterns of DNA supercoiling were observed in pat1Δ cells. Studies with pat1 alleles uncovered an evolutionarily conserved region within the central domain of Pat1 that is required for its association with centromeres, sister chromatid separation, and faithful chromosome segregation. Taken together, our data have uncovered a novel role for Pat1 in maintaining the structural integrity of centromeric chromatin to facilitate faithful chromosome segregation and proper kinetochore function.

Entities:  

Keywords:  Centromere; DNA topology; budding yeast; chromosome segregation; kinetochore

Mesh：

Substances：

Year:  2013        PMID： 23893485      PMCID： PMC3781966          DOI： 10.1534/genetics.113.155291

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  62 in total

1.  Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping.

Authors:  Guy R Pilkington; Roy Parker
Journal:  Mol Cell Biol       Date:  2007-12-17       Impact factor: 4.272

2.  Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes.

Authors:  Gaku Mizuguchi; Hua Xiao; Jan Wisniewski; M Mitchell Smith; Carl Wu
Journal:  Cell       Date:  2007-06-15       Impact factor: 41.582

3.  DNA topoisomerase II is a determinant of the tensile properties of yeast centromeric chromatin and the tension checkpoint.

Authors:  Tariq H Warsi; Michelle S Navarro; Jeff Bachant
Journal:  Mol Biol Cell       Date:  2008-08-13       Impact factor: 4.138

4.  Altered dosage and mislocalization of histone H3 and Cse4p lead to chromosome loss in Saccharomyces cerevisiae.

Authors:  Wei-Chun Au; Matthew J Crisp; Steven Z DeLuca; Oliver J Rando; Munira A Basrai
Journal:  Genetics       Date:  2008-05-05       Impact factor: 4.562

5.  Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore.

Authors:  Raymond Camahort; Bing Li; Laurence Florens; Selene K Swanson; Michael P Washburn; Jennifer L Gerton
Journal:  Mol Cell       Date:  2007-06-14       Impact factor: 17.970

6.  Centromere size and position in Candida albicans are evolutionarily conserved independent of DNA sequence heterogeneity.

Authors:  Prashant K Mishra; Mary Baum; John Carbon
Journal:  Mol Genet Genomics       Date:  2007-06-23       Impact factor: 3.291

7.  Analysis of P-body assembly in Saccharomyces cerevisiae.

Authors:  Daniela Teixeira; Roy Parker
Journal:  Mol Biol Cell       Date:  2007-04-11       Impact factor: 4.138

8.  Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins.

Authors:  Patrick Meraldi; Andrew D McAinsh; Esther Rheinbay; Peter K Sorger
Journal:  Genome Biol       Date:  2006-03-22       Impact factor: 13.583

9.  The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.

Authors:  Sujata Hajra; Santanu Kumar Ghosh; Makkuni Jayaram
Journal:  J Cell Biol       Date:  2006-09-11       Impact factor: 10.539

10.  Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae.

Authors:  Lars Boeckmann; Yoshimitsu Takahashi; Wei-Chun Au; Prashant K Mishra; John S Choy; Anthony R Dawson; May Y Szeto; Timothy J Waybright; Christopher Heger; Christopher McAndrew; Paul K Goldsmith; Timothy D Veenstra; Richard E Baker; Munira A Basrai
Journal:  Mol Biol Cell       Date:  2013-05-01       Impact factor: 4.138
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  12 in total

1.  The cytoplasmic mRNA degradation factor Pat1 is required for rRNA processing.

Authors:  Mridula Muppavarapu; Susanne Huch; Tracy Nissan
Journal:  RNA Biol       Date:  2016-02-26       Impact factor: 4.652

Review 2.  Centromeric heterochromatin: the primordial segregation machine.

Authors:  Kerry S Bloom
Journal:  Annu Rev Genet       Date:  2014-09-18       Impact factor: 16.830

3.  A 3D map of the yeast kinetochore reveals the presence of core and accessory centromere-specific histone.

Authors:  Julian Haase; Prashant K Mishra; Andrew Stephens; Rachel Haggerty; Cory Quammen; Russell M Taylor; Elaine Yeh; Munira A Basrai; Kerry Bloom
Journal:  Curr Biol       Date:  2013-09-26       Impact factor: 10.834

4.  Budding yeast CENP-ACse4 interacts with the N-terminus of Sgo1 and regulates its association with centromeric chromatin.

Authors:  Prashant K Mishra; Kriti S Thapa; Panyue Chen; Suyu Wang; Tony R Hazbun; Munira A Basrai
Journal:  Cell Cycle       Date:  2018-01-02       Impact factor: 4.534

5.  Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination.

Authors:  Prashant K Mishra; Jiasheng Guo; Lauren E Dittman; Julian Haase; Elaine Yeh; Kerry Bloom; Munira A Basrai
Journal:  Mol Biol Cell       Date:  2015-04-01       Impact factor: 4.138

6.  Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex.

Authors:  Ashis Chowdhury; Swathi Kalurupalle; Sundaresan Tharun
Journal:  RNA       Date:  2014-07-17       Impact factor: 4.942

7.  Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.

Authors:  Geetha S Hewawasam; Mark Mattingly; Swaminathan Venkatesh; Ying Zhang; Laurence Florens; Jerry L Workman; Jennifer L Gerton
Journal:  J Biol Chem       Date:  2014-09-02       Impact factor: 5.157

8.  Lessons learned from counting molecules: how to lure CENP-A into the kinetochore.

Authors:  Kristin C Scott; Kerry S Bloom
Journal:  Open Biol       Date:  2014-12       Impact factor: 6.411

9.  Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.

Authors:  Prashant K Mishra; Gudjon Olafsson; Lars Boeckmann; Timothy J Westlake; Ziad M Jowhar; Lauren E Dittman; Richard E Baker; Damien D'Amours; Peter H Thorpe; Munira A Basrai
Journal:  Mol Biol Cell       Date:  2019-02-06       Impact factor: 4.138

10.  Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis.

Authors:  Prashant K Mishra; Sultan Ciftci-Yilmaz; David Reynolds; Wei-Chun Au; Lars Boeckmann; Lauren E Dittman; Ziad Jowhar; Tejaswini Pachpor; Elaine Yeh; Richard E Baker; M Andrew Hoyt; Damien D'Amours; Kerry Bloom; Munira A Basrai
Journal:  Mol Biol Cell       Date:  2016-05-25       Impact factor: 4.138
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