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Literature DB >> 2388633

The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro.

Abstract

Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be detected in vitro but only under very stringent conditions.

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Year: 1990 PMID： 2388633 PMCID： PMC361124 DOI： 10.1128/mcb.10.9.4970-4973.1990

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

39 in total

1. Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters.

Authors: S T Smale; R Tjian
Journal: Mol Cell Biol Date: 1985-02 Impact factor: 4.272

2. Ribosomal RNA transcription: proteins and DNA sequences involved in preinitiation complex formation.

Authors: C T Iida; P Kownin; M R Paule
Journal: Proc Natl Acad Sci U S A Date: 1985-03 Impact factor: 11.205

3. Efficient transcription of a protein-coding gene from the RNA polymerase I promoter in transfected cells.

Authors: I Grummt; J A Skinner
Journal: Proc Natl Acad Sci U S A Date: 1985-02 Impact factor: 11.205

4. Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I.

Authors: I Grummt
Journal: Proc Natl Acad Sci U S A Date: 1982-11 Impact factor: 11.205

5. Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.

Authors: O Yamamoto; N Takakusa; Y Mishima; R Kominami; M Muramatsu
Journal: Proc Natl Acad Sci U S A Date: 1984-01 Impact factor: 11.205

6. A component of Drosophila RNA polymerase I promoter lies within the rRNA transcription unit.

Authors: B D Kohorn; P M Rae
Journal: Nature Date: 1983 Jul 14-20 Impact factor: 49.962

7. Regulation of human ribosomal RNA transcription.

Authors: R M Learned; S T Smale; M M Haltiner; R Tjian
Journal: Proc Natl Acad Sci U S A Date: 1983-06 Impact factor: 11.205

8. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.

Authors: M A Lopata; D W Cleveland; B Sollner-Webb
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

9. Transcription of Xenopus ribosomal RNA genes by RNA polymerase I in vitro.

Authors: J K Wilkinson; B Sollner-Webb
Journal: J Biol Chem Date: 1982-12-10 Impact factor: 5.157

10. Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I.

Authors: B Sollner-Webb; J A Wilkinson; J Roan; R H Reeder
Journal: Cell Date: 1983-11 Impact factor: 41.582

11 in total

Review 1. Survey and summary: transcription by RNA polymerases I and III.

Authors: M R Paule; R J White
Journal: Nucleic Acids Res Date: 2000-03-15 Impact factor: 16.971

2. Domains of the rat rDNA promoter must be aligned stereospecifically.

Authors: W Q Xie; L I Rothblum
Journal: Mol Cell Biol Date: 1992-03 Impact factor: 4.272

3. Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro.

Authors: J Saez-Vasquez; C S Pikaard
Journal: Proc Natl Acad Sci U S A Date: 1997-10-28 Impact factor: 11.205

The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro.

1. Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters.

2. Ribosomal RNA transcription: proteins and DNA sequences involved in preinitiation complex formation.

3. Efficient transcription of a protein-coding gene from the RNA polymerase I promoter in transfected cells.

4. Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I.

5. Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.

6. A component of Drosophila RNA polymerase I promoter lies within the rRNA transcription unit.

7. Regulation of human ribosomal RNA transcription.

8. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.

9. Transcription of Xenopus ribosomal RNA genes by RNA polymerase I in vitro.

10. Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I.

Review 1. Survey and summary: transcription by RNA polymerases I and III.

2. Domains of the rat rDNA promoter must be aligned stereospecifically.

3. Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro.

4. Analysis of the rat ribosomal DNA promoter: characterization of linker-scanning mutants and of the binding of UBF.

5. Identification of a sequence-specific protein binding the 5'-transcribed spacer of rat ribosomal genes.

Review 6. Expression of mouse and frog rRNA genes: transcription and processing.

7. Common DNA structural features exhibited by eukaryotic ribosomal gene promoters.

8. Stimulation of the mouse rRNA gene promoter by a distal spacer promoter.

9. Transcription from the rat 45S ribosomal DNA promoter does not require the factor UBF.

10. In vitro definition of the yeast RNA polymerase I promoter.