Literature DB >> 23878250

Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains.

Kimberly E Beatty1, Monique Williams, Brian L Carlson, Benjamin M Swarts, Robin M Warren, Paul D van Helden, Carolyn R Bertozzi.   

Abstract

Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.

Entities:  

Keywords:  Mycobacterium tuberculosis; chemical biology; enzyme assay; fluorescence; hydrolase

Mesh：

Substances：

Year:  2013        PMID： 23878250      PMCID： PMC3740907          DOI： 10.1073/pnas.1222041110

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


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