Literature DB >> 23877692

Characterization of the new AmpC β-lactamase FOX-8 reveals a single mutation, Phe313Leu, located in the R2 loop that affects ceftazidime hydrolysis.

Francisco José Pérez-Llarena1, Frédéric Kerff, Laura Zamorano, María Carmen Fernández, Maria Luz Nuñez, Elisenda Miró, Antonio Oliver, Ferran Navarro, Germán Bou.   

Abstract

A novel class C β-lactamase (FOX-8) was isolated from a clinical strain of Escherichia coli. The FOX-8 enzyme possessed a unique substitution (Phe313Leu) compared to FOX-3. Isogenic E. coli strains carrying FOX-8 showed an 8-fold reduction in resistance to ceftazidime relative to FOX-3. In a kinetic analysis, FOX-8 displayed a 33-fold reduction in kcat/Km for ceftazidime compared to FOX-3. In the FOX family of β-lactamases, the Phe313 residue located in the R2 loop affects ceftazidime hydrolysis and alters the phenotype of E. coli strains carrying this variant.

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Year:  2013        PMID： 23877692      PMCID： PMC3811397          DOI： 10.1128/AAC.00818-13

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  16 in total

1.  Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR.

Authors:  F Javier Pérez-Pérez; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

2.  Molecular characterization of FOX-4, a new AmpC-type plasmid-mediated beta-lactamase from an Escherichia coli strain isolated in Spain.

Authors:  G Bou; A Oliver; M Ojeda; C Monzón; J Martínez-Beltrán
Journal:  Antimicrob Agents Chemother       Date:  2000-09       Impact factor: 5.191

3.  Structural basis for the extended substrate spectrum of CMY-10, a plasmid-encoded class C beta-lactamase.

Authors:  Jae Young Kim; Ha Il Jung; Young Jun An; Jung Hun Lee; So Jung Kim; Seok Hoon Jeong; Kye Joon Lee; Pann-Ghill Suh; Heung-Soo Lee; Sang Hee Lee; Sun-Shin Cha
Journal:  Mol Microbiol       Date:  2006-05       Impact factor: 3.501

4.  A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.

Authors:  A Bauernfeind; S Wagner; R Jungwirth; I Schneider; D Meyer
Journal:  Antimicrob Agents Chemother       Date:  1997-09       Impact factor: 5.191

5.  Prevalence and molecular epidemiology of acquired AmpC β-lactamases and carbapenemases in Enterobacteriaceae isolates from 35 hospitals in Spain.

Authors:  E Miró; J Agüero; M N Larrosa; A Fernández; M C Conejo; G Bou; J J González-López; N Lara; L Martínez-Martínez; A Oliver; B Aracil; J Oteo; A Pascual; J Rodríguez-Baño; L Zamorano; F Navarro
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-09-07       Impact factor: 3.267

6.  Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants.

Authors:  M Gonzalez Leiza; J C Perez-Diaz; J Ayala; J M Casellas; J Martinez-Beltran; K Bush; F Baquero
Journal:  Antimicrob Agents Chemother       Date:  1994-09       Impact factor: 5.191

7.  Characterization of FOX-3, an AmpC-type plasmid-mediated beta-lactamase from an Italian isolate of Klebsiella oxytoca.

Authors:  A Marchese; G Arlet; G C Schito; P H Lagrange; A Philippon
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191

8.  Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria.

Authors:  S L Liu; A Hessel; K E Sanderson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-15       Impact factor: 11.205

9.  Sequence analysis and biochemical characterisation of chromosomal CAV-1 (Aeromonas caviae), the parental cephalosporinase of plasmid-mediated AmpC 'FOX' cluster.

Authors:  Thierry Fosse; Chantal Giraud-Morin; Isabelle Madinier; Roger Labia
Journal:  FEMS Microbiol Lett       Date:  2003-05-16       Impact factor: 2.742

10.  Acquisition and diffusion of bla CTX-M-9 gene by R478-IncHI2 derivative plasmids.

Authors:  Aurora García; Ferran Navarro; Elisenda Miró; Laura Villa; Beatriz Mirelis; Pere Coll; Alessandra Carattoli
Journal:  FEMS Microbiol Lett       Date:  2007-03-28       Impact factor: 2.742
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  4 in total

1.  Mobile genetic elements related to the diffusion of plasmid-mediated AmpC β-lactamases or carbapenemases from Enterobacteriaceae: findings from a multicenter study in Spain.

Authors:  L Zamorano; E Miró; C Juan; L Gómez; G Bou; J J González-López; L Martínez-Martínez; B Aracil; M C Conejo; A Oliver; F Navarro
Journal:  Antimicrob Agents Chemother       Date:  2015-06-15       Impact factor: 5.191

Review 2.  Class C β-Lactamases: Molecular Characteristics.

Authors:  Alain Philippon; Guillaume Arlet; Roger Labia; Bogdan I Iorga
Journal:  Clin Microbiol Rev       Date:  2022-04-18       Impact factor: 50.129

3.  Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella isolates from intensive care unit patients and the utility of whole-genome sequencing in health care settings.

Authors:  Tracy H Hazen; LiCheng Zhao; Mallory A Boutin; Angela Stancil; Gwen Robinson; Anthony D Harris; David A Rasko; J Kristie Johnson
Journal:  Antimicrob Agents Chemother       Date:  2014-06-09       Impact factor: 5.191

4.  Analysis of the Structure and Function of FOX-4 Cephamycinase.

Authors:  S T Lefurgy; V N Malashkevich; J T Aguilan; E Nieves; E C Mundorff; B Biju; M A Noel; R Toro; D Baiwir; K M Papp-Wallace; S C Almo; J-M Frere; G Bou; R A Bonomo
Journal:  Antimicrob Agents Chemother       Date:  2015-11-02       Impact factor: 5.191
  4 in total

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