ETHNOPHARMACOLOGICAL RELEVANCE: Rhizoma coptidis (RC) has been used as a remedy for inflammation-related diseases in traditional medicine. Although it is known to have anti-inflammatory activities, its mechanism of action on lipopolysaccharide (LPS)-induced inflammation has not yet been identified in detail. AIM OF THE STUDY: This study was designed to assess the beneficial effects of pretreatment with RC in ameliorating LPS-induced liver inflammation. MATERIALS AND METHODS: Mice were orally administered RC (500, 1000 mg/kg) for three days in a row. 1h after the last RC administration, the mice were intraperitoneally injected with LPS (35 mg/kg). After treatment, histological alterations and inflammatory factor levels in the liver and proinflammatory cytokines in the serum associated with inflammation were examined. RESULTS: We found that pretreatment with RC (500 and 1000 mg/kg) exerted a significant protective effect by attenuating liver histopathological changes in endotoxemic mice. The results also demonstrated that RC suppressed secretion of LPS-stimulated pro-inflammatory cytokines, such as interleukin-6 (IL-6). Furthermore, RC inhibited LPS-mediated nuclear factor (NF)-κB activation via the prevention of IκB-α phosphorylation, as well as the phosphorylation of ERK1/2, JNK, and p38 MAPKs. These results were associated with decreases in the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (I-NOS). CONCLUSIONS: The results presented here clearly demonstrate that RC could significantly protect mice against LPS-induced acute liver injury.
ETHNOPHARMACOLOGICAL RELEVANCE: Rhizoma coptidis (RC) has been used as a remedy for inflammation-related diseases in traditional medicine. Although it is known to have anti-inflammatory activities, its mechanism of action on lipopolysaccharide (LPS)-induced inflammation has not yet been identified in detail. AIM OF THE STUDY: This study was designed to assess the beneficial effects of pretreatment with RC in ameliorating LPS-induced liver inflammation. MATERIALS AND METHODS:Mice were orally administered RC (500, 1000 mg/kg) for three days in a row. 1h after the last RC administration, the mice were intraperitoneally injected with LPS (35 mg/kg). After treatment, histological alterations and inflammatory factor levels in the liver and proinflammatory cytokines in the serum associated with inflammation were examined. RESULTS: We found that pretreatment with RC (500 and 1000 mg/kg) exerted a significant protective effect by attenuating liver histopathological changes in endotoxemic mice. The results also demonstrated that RC suppressed secretion of LPS-stimulated pro-inflammatory cytokines, such as interleukin-6 (IL-6). Furthermore, RC inhibited LPS-mediated nuclear factor (NF)-κB activation via the prevention of IκB-α phosphorylation, as well as the phosphorylation of ERK1/2, JNK, and p38 MAPKs. These results were associated with decreases in the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (I-NOS). CONCLUSIONS: The results presented here clearly demonstrate that RC could significantly protect mice against LPS-induced acute liver injury.
Authors: Sang Mi Park; Byung-Gu Min; Ji Yun Jung; Kyung Hwan Jegal; Chul Won Lee; Kwang Youn Kim; Young Woo Kim; Youn-Woong Choi; Il Je Cho; Sae Kwang Ku; Sang Chan Kim Journal: BMC Complement Altern Med Date: 2018-01-19 Impact factor: 3.659
Authors: Goohyeon Hong; Yu-Il Kim; Seoung Ju Park; Sung Yong Lee; Jin Woo Kim; Seong Hoon Yoon; Keu Sung Lee; Min Kwang Byun; Hak-Ryul Kim; Jaeho Chung Journal: Int J Environ Res Public Health Date: 2021-04-12 Impact factor: 3.390