The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.
Authors: Ondřej Kaplan; Karel Bezouška; Ondřej Plíhal; Rüdiger Ettrich; Natallia Kulik; Ondřej Vaněk; Daniel Kavan; Oldřich Benada; Anna Malandra; Ondřej Sveda; Alicja B Veselá; Anna Rinágelová; Kristýna Slámová; Maria Cantarella; Jürgen Felsberg; Jarmila Dušková; Jan Dohnálek; Michael Kotik; Vladimír Křen; Ludmila Martínková Journal: BMC Biotechnol Date: 2011-01-06 Impact factor: 2.563