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Literature DB >> 23868592

Purification of native Arp2/3 complex from bovine thymus.

Lynda K Doolittle¹, Michael K Rosen, Shae B Padrick.

Abstract

The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.

Entities: Chemical

Mesh：

Substances：

Year: 2013 PMID： 23868592 PMCID： PMC3994882 DOI： 10.1007/978-1-62703-538-5_14

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

47 in total

1. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins.

Authors: T P Loisel; R Boujemaa; D Pantaloni; M F Carlier
Journal: Nature Date: 1999-10-07 Impact factor: 49.962

2. Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity.

Authors: H Gournier; E D Goley; H Niederstrasser; T Trinh; M D Welch
Journal: Mol Cell Date: 2001-11 Impact factor: 17.970

Review 3. Cellular motility driven by assembly and disassembly of actin filaments.

Authors: Thomas D Pollard; Gary G Borisy
Journal: Cell Date: 2003-02-21 Impact factor: 41.582

4. Pathway of actin filament branch formation by Arp2/3 complex.

Authors: Christopher C Beltzner; Thomas D Pollard
Journal: J Biol Chem Date: 2007-12-29 Impact factor: 5.157

5. Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis.

Authors: Karen M Daugherty; Bruce L Goode
Journal: J Biol Chem Date: 2008-03-31 Impact factor: 5.157

6. Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex.

Authors: Orkun Akin; R Dyche Mullins
Journal: Cell Date: 2008-05-30 Impact factor: 41.582

7. Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins.

Authors: L Blanchoin; K J Amann; H N Higgs; J B Marchand; D A Kaiser; T D Pollard
Journal: Nature Date: 2000-04-27 Impact factor: 49.962

8. Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization.

Authors: H N Higgs; L Blanchoin; T D Pollard
Journal: Biochemistry Date: 1999-11-16 Impact factor: 3.162

9. Measurement and analysis of in vitro actin polymerization.

Authors: Lynda K Doolittle; Michael K Rosen; Shae B Padrick
Journal: Methods Mol Biol Date: 2013

10. The structural basis of actin filament branching by the Arp2/3 complex.

Authors: Isabelle Rouiller; Xiao-Ping Xu; Kurt J Amann; Coumaran Egile; Stephan Nickell; Daniela Nicastro; Rong Li; Thomas D Pollard; Niels Volkmann; Dorit Hanein
Journal: J Cell Biol Date: 2008-03-03 Impact factor: 10.539

7 in total

1. Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation.

Authors: Benjamin A Smith; Shae B Padrick; Lynda K Doolittle; Karen Daugherty-Clarke; Ivan R Corrêa; Ming-Qun Xu; Bruce L Goode; Michael K Rosen; Jeff Gelles
Journal: Elife Date: 2013-09-03 Impact factor: 8.140

Purification of native Arp2/3 complex from bovine thymus.

1. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins.

2. Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity.

Review 3. Cellular motility driven by assembly and disassembly of actin filaments.

4. Pathway of actin filament branch formation by Arp2/3 complex.

5. Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis.

6. Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex.

7. Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins.

8. Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization.

9. Measurement and analysis of in vitro actin polymerization.

10. The structural basis of actin filament branching by the Arp2/3 complex.

1. Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation.

2. Stoichiometry controls activity of phase-separated clusters of actin signaling proteins.

3. Actin Cross-Linking Toxin Is a Universal Inhibitor of Tandem-Organized and Oligomeric G-Actin Binding Proteins.

4. The molecular mechanism of load adaptation by branched actin networks.

5. WH2 and proline-rich domains of WASP-family proteins collaborate to accelerate actin filament elongation.

6. Reconstitution of Phase-Separated Signaling Clusters and Actin Polymerization on Supported Lipid Bilayers.

7. Purification of Arp2/3 complex from Saccharomyces cerevisiae.