| Literature DB >> 23867288 |
Ming Xue1, Linlin Zhu, Yiming Meng, Li Wang, Haiyan Sun, Fen Wang, Enhu Wang, Fengping Shan.
Abstract
IFN-γ is a cytokine that plays crucial role in innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFN-γ is also a key activator of macrophages [1,2]. In the present study, we studied detailed modulation of IFN-γ on phenotypic and functional maturation of murine bone marrow derived dendritic cells (BMDCs). Phenotypic and functional maturation of BMDCs was evaluated by light microscope, flow cytometry(FCM), transmission electron microscopy (TEM), cytochemistry method, acid phosphatase activity(ACP), FITC-dextran bio-assay and enzyme linked immunosorbent assay (ELISA). We elucidated that IFN-γ up-regulated the expression of MHC II, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-α secreted by BMDCs. It is therefore confirmed that IFN-γ can effectively promote the maturation of BMDCs. Our study provides more evidence and rationale on future application of IFN-γ for enhancing host immunity.Entities:
Keywords: 3, 3′-diaminobenzidine; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ACP; APCs; BMDCs; Bone marrow derived dendritic cells; CTL; DAB; FCM; GM-CSF; IFN-γ; IL-4; Immune system; Interferon-gamma; LPS; MACS; MTT; Maturation; Modulation; TEM; acidic phosphatase; antigen presenting cells; bone marrow derived dendritic cells; cytotoxic lymphocyte; flow cytometry; granulocyte macrophage colony stimulating factor; interferon-gamma; interleukin-4; lipopolysaccharide; magnetic activated cell sorting; transmission electron microscopy
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Year: 2013 PMID： 23867288 DOI： 10.1016/j.intimp.2013.07.002
Source DB: PubMed Journal: Int Immunopharmacol ISSN： 1567-5769 Impact factor: 4.932