| Literature DB >> 25622186 |
Xiaofang Hu1, Yan Cao, Yiming Meng, Mingxiao Hou.
Abstract
IL-2 is a pleiotropic cytokine produced by T cell after antigen activation of T cell and it is so called T cell growth factor. A large number of documents suggest that Il-2 plays pivotal roles in the immune response and now Il-2 is an approved drug being used for various kinds of diseases such as cancer and dermatitis. (1) The aim of present exploration was to look at effect of IL-2 on structural, phenotypic and functional maturation of murine BMDCs. The structural and phenotypic maturation of BMDCs under influence of IL-2 were evaluated by light microscope and flow cytometry (FCM). The functional maturation of BMDCs was confirmed by cytochemistry assay, FITC-dextran, acid phosphatase (ACP) activity, bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IL-2 up-regulated the expression of key surface markers such as: CD80, CD83, CD86, CD40 and MHC II molecules on BMDCs, down-regulated phagocytosis activity, induced more production of IL-12 and TNF-α secreted by BMDCs. Therefore it can be concluded that IL-2 effectively enhance the maturation of BMDCs. Our results provide direct evidence to support IL-2 would be used as a potent adjuvant in preparation of DC-based vaccines, as well as an immune remedy for cancer situation.Entities:
Keywords: ACP, Acidic phosphatase; APCs, LPS; BMDCs, Bone marrow derived dendritic cells; Bone marrow derived dendritic cells; CTL, Cytotoxic lymphocyte; DAB 3 3′-diaminobenzidine; FCM, Fow cytometry; IL-2, Interlukin-2; Interlukin-2; Lipopolysaccharide; MACS, Magnetic activated cell sorting; MTT 3-(4 5-Dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide; TEM, Transmission electron microscopy; maturation; surface molecules
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Year: 2015 PMID： 25622186 PMCID： PMC4514260 DOI： 10.1080/21645515.2015.1009336
Source DB: PubMed Journal: Hum Vaccin Immunother ISSN： 2164-5515 Impact factor: 3.452