Literature DB >> 23861344

A facile method for preparation of tailored scaffolds for DNA-origami.

Michael Erkelenz1, Dennis M Bauer, Rebecca Meyer, Christos Gatsogiannis, Stefan Raunser, Barbara Saccà, Christof M Niemeyer.   

Abstract

A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords:  DNA; bacterial cloning; nanostructures; self-assembly

Mesh：

Substances：

Year:  2013        PMID： 23861344     DOI： 10.1002/smll.201300701

Source DB:  PubMed          Journal:  Small        ISSN： 1613-6810            Impact factor:   13.281


  10 in total

1.  Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions.

Authors:  Andreas Sprengel; Pascal Lill; Pierre Stegemann; Kenny Bravo-Rodriguez; Elisa-C Schöneweiß; Melisa Merdanovic; Daniel Gudnason; Mikayel Aznauryan; Lisa Gamrad; Stephan Barcikowski; Elsa Sanchez-Garcia; Victoria Birkedal; Christos Gatsogiannis; Michael Ehrmann; Barbara Saccà
Journal:  Nat Commun       Date:  2017-02-16       Impact factor: 14.919

2.  Protein-Functionalized DNA Nanostructures as Tools to Control Transcription in Zebrafish Embryos.

Authors:  Alessandro Angelin; Olivier Kassel; Sepand Rastegar; Uwe Strähle; Christof M Niemeyer
Journal:  ChemistryOpen       Date:  2016-12-28       Impact factor: 2.911

3.  Construction of a novel phagemid to produce custom DNA origami scaffolds.

Authors:  Parsa M Nafisi; Tural Aksel; Shawn M Douglas
Journal:  Synth Biol (Oxf)       Date:  2018-08-09

4.  Intercalating Electron Dyes for TEM Visualization of DNA at the Single-Molecule Level.

Authors:  Yoones Kabiri; Alessandro Angelin; Ishtiaq Ahmed; Hatice Mutlu; Jens Bauer; Christof M Niemeyer; Henny Zandbergen; Cees Dekker
Journal:  Chembiochem       Date:  2019-02-07       Impact factor: 3.164

Review 5.  Synthesis of DNA Origami Scaffolds: Current and Emerging Strategies.

Authors:  Joshua Bush; Shrishti Singh; Merlyn Vargas; Esra Oktay; Chih-Hsiang Hu; Remi Veneziano
Journal:  Molecules       Date:  2020-07-26       Impact factor: 4.411

6.  One-Pot Synthesis of Defined-Length ssDNA for Multiscaffold DNA Origami.

Authors:  Willem E M Noteborn; Leoni Abendstein; Thomas H Sharp
Journal:  Bioconjug Chem       Date:  2020-12-13       Impact factor: 4.774

7.  Topogami: Topologically Linked DNA Origami.

Authors:  Yusuke Sakai; Gerrit D Wilkens; Karol Wolski; Szczepan Zapotoczny; Jonathan G Heddle
Journal:  ACS Nanosci Au       Date:  2021-11-12

8.  Simulations of DNA-Origami Self-Assembly Reveal Design-Dependent Nucleation Barriers.

Authors:  Alexander Cumberworth; Daan Frenkel; Aleks Reinhardt
Journal:  Nano Lett       Date:  2022-08-29       Impact factor: 12.262

9.  In vitro synthesis of gene-length single-stranded DNA.

Authors:  Rémi Veneziano; Tyson R Shepherd; Sakul Ratanalert; Leila Bellou; Chaoqun Tao; Mark Bathe
Journal:  Sci Rep       Date:  2018-04-25       Impact factor: 4.379

Review 10.  Current and Emerging Methods for the Synthesis of Single-Stranded DNA.

Authors:  Min Hao; Jianjun Qiao; Hao Qi
Journal:  Genes (Basel)       Date:  2020-01-21       Impact factor: 4.096
  10 in total

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