BACKGROUND: We have found that the expression of the novel focal adhesion protein kindlin-2 had a significant positive correlation with poor survival in gastric cancer. However, the mechanism by which kindlin-2 acts in gastric cancer warrants further evaluation. METHODS: Kindlin-2 mRNA expression in gastric cancer cell lines was measured by realtime RT-PCR under normal and hypoxic conditions. Cell proliferation, apoptosis, cell cycle, tumor adhesion, cell invasion ability, and phosphorylation of integrin β1 and β3 proteins were measured to assess the influence of kindlin-2 on the malignant behavior of gastric cancer cells. RESULTS: Kindlin-2 mRNA expression was highest in the distant metastasis gastric cancer cell line Hs-746T. Cell proliferation, adhesion with endothelium and collagen IV, invasion rate, and angiogenesis genes expression, as well as phosphorylation of integrin β1 and β3 in Hs-746T, were decreased significantly after kindlin-2 downregulation, but there was no change in apoptosis and cell cycle. CONCLUSIONS: Kindlin-2 might promote the invasion of gastric cancer cells through enhancing proliferation and adhesion by the phosphorylation of integrin β1 and β3.
BACKGROUND: We have found that the expression of the novel focal adhesion protein kindlin-2 had a significant positive correlation with poor survival in gastric cancer. However, the mechanism by which kindlin-2 acts in gastric cancer warrants further evaluation. METHODS:Kindlin-2 mRNA expression in gastric cancer cell lines was measured by realtime RT-PCR under normal and hypoxic conditions. Cell proliferation, apoptosis, cell cycle, tumor adhesion, cell invasion ability, and phosphorylation of integrin β1 and β3 proteins were measured to assess the influence of kindlin-2 on the malignant behavior of gastric cancer cells. RESULTS:Kindlin-2 mRNA expression was highest in the distant metastasis gastric cancer cell line Hs-746T. Cell proliferation, adhesion with endothelium and collagen IV, invasion rate, and angiogenesis genes expression, as well as phosphorylation of integrin β1 and β3 in Hs-746T, were decreased significantly after kindlin-2 downregulation, but there was no change in apoptosis and cell cycle. CONCLUSIONS:Kindlin-2 might promote the invasion of gastric cancer cells through enhancing proliferation and adhesion by the phosphorylation of integrin β1 and β3.
Authors: Nikolaos Vassos; Tilman Rau; Susanne Merkel; Fabian Feiersinger; Carol I Geppert; Michael Stürzl; Werner Hohenberger; Roland S Croner Journal: Int J Clin Exp Pathol Date: 2013-12-15
Authors: Chiea Chuen Khor; Tan Do; Hongyan Jia; Masakazu Nakano; Ronnie George; Khaled Abu-Amero; Roopam Duvesh; Li Jia Chen; Zheng Li; Monisha E Nongpiur; Shamira A Perera; Chunyan Qiao; Hon-Tym Wong; Hiroshi Sakai; Mônica Barbosa de Melo; Mei-Chin Lee; Anita S Chan; Yaakub Azhany; Thi Lam Huong Dao; Yoko Ikeda; Rodolfo A Perez-Grossmann; Tomasz Zarnowski; Alexander C Day; Jost B Jonas; Pancy O S Tam; Tuan Anh Tran; Humaira Ayub; Farah Akhtar; Shazia Micheal; Paul T K Chew; Leyla A Aljasim; Tanuj Dada; Tam Thi Luu; Mona S Awadalla; Naris Kitnarong; Boonsong Wanichwecharungruang; Yee Yee Aung; Jelinar Mohamed-Noor; Saravanan Vijayan; Sripriya Sarangapani; Rahat Husain; Aliza Jap; Mani Baskaran; David Goh; Daniel H Su; Huaizhou Wang; Vernon K Yong; Leonard W Yip; Tuyet Bach Trinh; Manchima Makornwattana; Thanh Thu Nguyen; Edgar U Leuenberger; Ki-Ho Park; Widya Artini Wiyogo; Rajesh S Kumar; Celso Tello; Yasuo Kurimoto; Suman S Thapa; Kessara Pathanapitoon; John F Salmon; Yong Ho Sohn; Antonio Fea; Mineo Ozaki; Jimmy S M Lai; Visanee Tantisevi; Chaw Chaw Khaing; Takanori Mizoguchi; Satoko Nakano; Chan-Yun Kim; Guangxian Tang; Sujie Fan; Renyi Wu; Hailin Meng; Thi Thuy Giang Nguyen; Tien Dat Tran; Morio Ueno; Jose Maria Martinez; Norlina Ramli; Yin Mon Aung; Rigo Daniel Reyes; Stephen A Vernon; Seng Kheong Fang; Zhicheng Xie; Xiao Yin Chen; Jia Nee Foo; Kar Seng Sim; Tina T Wong; Desmond T Quek; Rengaraj Venkatesh; Srinivasan Kavitha; Subbiah R Krishnadas; Nagaswamy Soumittra; Balekudaru Shantha; Boon-Ang Lim; Jeanne Ogle; José P C de Vasconcellos; Vital P Costa; Ricardo Y Abe; Bruno B de Souza; Chelvin C Sng; Maria C Aquino; Ewa Kosior-Jarecka; Guillermo Barreto Fong; Vania Castro Tamanaja; Ricardo Fujita; Yuzhen Jiang; Naushin Waseem; Sancy Low; Huan Nguyen Pham; Sami Al-Shahwan; E Randy Craven; Muhammad Imran Khan; Rrima Dada; Kuldeep Mohanty; Muneeb A Faiq; Alex W Hewitt; Kathryn P Burdon; Eng Hui Gan; Anuwat Prutthipongsit; Thipnapa Patthanathamrongkasem; Mary Ann T Catacutan; Irene R Felarca; Chona S Liao; Emma Rusmayani; Vira Wardhana Istiantoro; Giulia Consolandi; Giulia Pignata; Carlo Lavia; Prin Rojanapongpun; Lerprat Mangkornkanokpong; Sunee Chansangpetch; Jonathan C H Chan; Bonnie N K Choy; Jennifer W H Shum; Hlaing May Than; Khin Thida Oo; Aye Thi Han; Victor H Yong; Xiao-Yu Ng; Shuang Ru Goh; Yaan Fun Chong; Martin L Hibberd; Mark Seielstad; Eileen Png; Sarah J Dunstan; Nguyen Van Vinh Chau; Jinxin Bei; Yi Xin Zeng; Abhilasha Karkey; Buddha Basnyat; Francesca Pasutto; Daniela Paoli; Paolo Frezzotti; Jie Jin Wang; Paul Mitchell; John H Fingert; R Rand Allingham; Michael A Hauser; Soon Thye Lim; Soo Hong Chew; Richard P Ebstein; Anavaj Sakuntabhai; Kyu Hyung Park; Jeeyun Ahn; Greet Boland; Harm Snippe; Richard Stead; Raquel Quino; Su Nyunt Zaw; Urszula Lukasik; Rohit Shetty; Mimiwati Zahari; Hyoung Won Bae; Nay Lin Oo; Toshiaki Kubota; Anita Manassakorn; Wing Lau Ho; Laura Dallorto; Young Hoon Hwang; Christine A Kiire; Masako Kuroda; Zeiras Eka Djamal; Jovell Ian M Peregrino; Arkasubhra Ghosh; Jin Wook Jeoung; Tung S Hoan; Nuttamon Srisamran; Thayanithi Sandragasu; Saw Htoo Set; Vi Huyen Doan; Shomi S Bhattacharya; Ching-Lin Ho; Donald T Tan; Ramanjit Sihota; Seng-Chee Loon; Kazuhiko Mori; Shigeru Kinoshita; Anneke I den Hollander; Raheel Qamar; Ya-Xing Wang; Yik Y Teo; E-Shyong Tai; Curt Hartleben-Matkin; David Lozano-Giral; Seang Mei Saw; Ching-Yu Cheng; Juan C Zenteno; Chi Pui Pang; Huong T T Bui; Owen Hee; Jamie E Craig; Deepak P Edward; Michiko Yonahara; Jamil Miguel Neto; Maria L Guevara-Fujita; Liang Xu; Robert Ritch; Ahmad Tajudin Liza-Sharmini; Tien Y Wong; Saleh Al-Obeidan; Nhu Hon Do; Periasamy Sundaresan; Clement C Tham; Paul J Foster; Lingam Vijaya; Kei Tashiro; Eranga N Vithana; Ningli Wang; Tin Aung Journal: Nat Genet Date: 2016-04-04 Impact factor: 38.330