| Literature DB >> 23846655 |
Debashish Ray1, Hilal Kazan, Kate B Cook, Matthew T Weirauch, Hamed S Najafabadi, Xiao Li, Serge Gueroussov, Mihai Albu, Hong Zheng, Ally Yang, Hong Na, Manuel Irimia, Leah H Matzat, Ryan K Dale, Sarah A Smith, Christopher A Yarosh, Seth M Kelly, Behnam Nabet, Desirea Mecenas, Weimin Li, Rakesh S Laishram, Mei Qiao, Howard D Lipshitz, Fabio Piano, Anita H Corbett, Russ P Carstens, Brendan J Frey, Richard A Anderson, Kristen W Lynch, Luiz O F Penalva, Elissa P Lei, Andrew G Fraser, Benjamin J Blencowe, Quaid D Morris, Timothy R Hughes.
Abstract
RNA-binding proteins are key regulators of gene expression, yet only a small fraction have been functionally characterized. Here we report a systematic analysis of the RNA motifs recognized by RNA-binding proteins, encompassing 205 distinct genes from 24 diverse eukaryotes. The sequence specificities of RNA-binding proteins display deep evolutionary conservation, and the recognition preferences for a large fraction of metazoan RNA-binding proteins can thus be inferred from their RNA-binding domain sequence. The motifs that we identify in vitro correlate well with in vivo RNA-binding data. Moreover, we can associate them with distinct functional roles in diverse types of post-transcriptional regulation, enabling new insights into the functions of RNA-binding proteins both in normal physiology and in human disease. These data provide an unprecedented overview of RNA-binding proteins and their targets, and constitute an invaluable resource for determining post-transcriptional regulatory mechanisms in eukaryotes.Entities:
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Year: 2013 PMID： 23846655 PMCID： PMC3929597 DOI： 10.1038/nature12311
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962