BACKGROUND: The measurement and detection of viremia and antigenemia in sera have been used as a marker of risk for dengue disease severity and diagnosis. However, evidence exists suggesting that levels of viremia and antigenemia are affected by the presence of specific antibodies. OBJECTIVE: To compare viral load and circulating NS1 levels in sera from patients positive or negative for dengue specific IgM antibodies. STUDY DESIGN: Three hundred and eighty serum samples were analyzed for viral load using qRT-PCR and for levels of circulating NS1 and the presence of specific antibodies using commercial EIAs. RESULTS AND CONCLUSION: Comparison of viremia levels in sera from patients positive or negative for dengue IgM antibodies showed that viral load was significantly higher (p≤0.0001) in patients negative for IgM antibodies. In contrast, levels of circulating NS1 were found unaffected by the presence of IgM (p=0.0038). Thus, dengue virus specific IgM antibodies in sera seem to be a strong negative modulator of viremia levels in patient's sera.
BACKGROUND: The measurement and detection of viremia and antigenemia in sera have been used as a marker of risk for dengue disease severity and diagnosis. However, evidence exists suggesting that levels of viremia and antigenemia are affected by the presence of specific antibodies. OBJECTIVE: To compare viral load and circulating NS1 levels in sera from patients positive or negative for dengue specific IgM antibodies. STUDY DESIGN: Three hundred and eighty serum samples were analyzed for viral load using qRT-PCR and for levels of circulating NS1 and the presence of specific antibodies using commercial EIAs. RESULTS AND CONCLUSION: Comparison of viremia levels in sera from patients positive or negative for dengue IgM antibodies showed that viral load was significantly higher (p≤0.0001) in patients negative for IgM antibodies. In contrast, levels of circulating NS1 were found unaffected by the presence of IgM (p=0.0038). Thus, dengue virus specific IgM antibodies in sera seem to be a strong negative modulator of viremia levels in patient's sera.
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