Literature DB >> 23836674

Semisynthetic lipidated LC3 protein mediates membrane fusion.

Aimin Yang1, Yanping Li, Supansa Pantoom, Gemma Triola, Yao-Wen Wu.   

Abstract

All together: Lipidated LC3 has been synthesized by expressed protein ligation. A TEV-cleavable MBP tag was employed to facilitate ligation under folding conditions and to solubilize the lipidated protein. The synthetic LC3-phosphatidylethanolamine (PE) mediates membrane tethering and fusion at the physiological concentration of PE, and could be a useful tool for autophagy studies.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  LC3; autophagy; expressed protein ligation; lipidated proteins; membrane fusion; protein synthesis

Mesh：

Substances：

Year:  2013        PMID： 23836674     DOI： 10.1002/cbic.201300344

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  12 in total

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7.  Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins.

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9.  Distinct Mechanisms for Processing Autophagy Protein LC3-PE by RavZ and ATG4B.

Authors:  Aimin Yang; Supansa Pantoom; Yao-Wen Wu
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