OBJECTIVES/HYPOTHESIS: In this study, a bioengineered trachea composed of autologous chondrocytes was developed, and its effect on cartilaginous regeneration was evaluated by implantation into tracheal defects in rabbits. STUDY DESIGN: Prospective controlled trial in an animal model. METHODS: The tracheal prosthesis used in this study was composed of polypropylene (the frame) and collagen sponge (the scaffold). Chondrocytes were harvested from the costal cartilage of rabbits and seeded into the tracheal prosthesis. The bioengineered trachea, consisting of the tracheal prosthesis with chondrocytes, was implanted into surgically created tracheal defects of rabbits in the bioengineered group, and a tracheal prosthesis without chondrocytes was implanted in the control group. RESULTS: After implantation, the presence of regenerated cartilage was observed in the bioengineered trachea but not in the tracheal prosthesis without chondrocytes. CONCLUSIONS: It was confirmed in this study that the implanted chondrocytes proliferated in an appropriate portion of the tracheal defect and that the partially resected tracheal cartilage was repaired with regenerated cartilaginous tissue into a ring-shaped form as a whole. These results demonstrate the feasibility of cartilaginous regeneration using a bioengineered trachea with autologous chondrocytes.
OBJECTIVES/HYPOTHESIS: In this study, a bioengineered trachea composed of autologous chondrocytes was developed, and its effect on cartilaginous regeneration was evaluated by implantation into tracheal defects in rabbits. STUDY DESIGN: Prospective controlled trial in an animal model. METHODS: The tracheal prosthesis used in this study was composed of polypropylene (the frame) and collagen sponge (the scaffold). Chondrocytes were harvested from the costal cartilage of rabbits and seeded into the tracheal prosthesis. The bioengineered trachea, consisting of the tracheal prosthesis with chondrocytes, was implanted into surgically created tracheal defects of rabbits in the bioengineered group, and a tracheal prosthesis without chondrocytes was implanted in the control group. RESULTS: After implantation, the presence of regenerated cartilage was observed in the bioengineered trachea but not in the tracheal prosthesis without chondrocytes. CONCLUSIONS: It was confirmed in this study that the implanted chondrocytes proliferated in an appropriate portion of the tracheal defect and that the partially resected tracheal cartilage was repaired with regenerated cartilaginous tissue into a ring-shaped form as a whole. These results demonstrate the feasibility of cartilaginous regeneration using a bioengineered trachea with autologous chondrocytes.
Authors: Liping Zhao; Sumati Sundaram; Andrew V Le; Angela H Huang; Jiasheng Zhang; Go Hatachi; Arkadi Beloiartsev; Michael G Caty; Tai Yi; Katherine Leiby; Ashley Gard; Mehmet H Kural; Liqiong Gui; Kevin A Rocco; Amogh Sivarapatna; Elizabeth Calle; Allison Greaney; Luca Urbani; Panagiotis Maghsoudlou; Alan Burns; Paolo DeCoppi; Laura E Niklason Journal: Tissue Eng Part A Date: 2016-09 Impact factor: 3.845
Authors: Elizabeth F Maughan; Robert E Hynds; Toby J Proctor; Sam M Janes; Martin Elliott; Martin A Birchall; Mark W Lowdell; Paolo De Coppi Journal: Curr Stem Cell Rep Date: 2017-10-26
Authors: Nelson Bergonse Neto; Lianna Ferrari Jorge; Julio C Francisco; Bruna Olandoski Erbano; Barbara Evelin Gonçalves Barboza; Larissa Luvison Gomes da Silva; Marcia Olandoski; Katherine Athayde Teixeira de Carvalho; Luiz Felipe Pinho Moreira; Jose Rocha Faria Neto; Eltyeb Abdelwahid; Luiz Cesar Guarita-Souza Journal: Stem Cells Int Date: 2018-02-26 Impact factor: 5.443