| Literature DB >> 23818676 |
Michael F Carey, Craig L Peterson, Stephen T Smale.
Abstract
In an electrophoretic mobility-shift assay (EMSA, or simply "gel shift"), a (32)P-labeled DNA fragment containing a specific DNA site is incubated with a cognate DNA-binding protein. The protein-DNA complexes are separated from free (unbound) DNA by electrophoresis through a nondenaturing polyacrylamide gel. The protein retards the mobility of the DNA fragments to which it binds. Thus, the free DNA will migrate faster than the DNA-protein complex. An image of the gel is used to reveal the positions of the free and bound radiolabeled DNAs.Entities:
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Year: 2013 PMID： 23818676 DOI： 10.1101/pdb.prot075861
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095