| Literature DB >> 23816563 |
Minzhi Peng1, Li Liu, Minyan Jiang, Cuili Liang, Xiaoyuan Zhao, Yanna Cai, Huiying Sheng, Zhiying Ou, Hong Luo.
Abstract
Measurement of carnitine and acylcarnitines in plasma is important in diagnosis of fatty acid β-oxidation disorders and organic acidemia. The usual method uses flow injection tandem mass spectrometry (FIA-MS/MS), which has limitations. A rapid and more accurate method was developed to be used for high-risk screening and diagnosis. Carnitine and acylcarnitines were separated by hydrophilic interaction liquid chromatography (HILIC) without derivatization and detected with a QTRAP MS/MS System. Total analysis time was 9.0min. The imprecision of within- and between-run were less than 6% and 17%, respectively. Recoveries were in the range of 85-110% at three concentrations. Some acylcarnitine isomers could be separated, such as dicarboxylic and hydroxyl acylcarnitines. The method could also separate interferent to avoid false positive results. 216 normal samples and 116 patient samples were detected with the validated method, and 49 patients were identified with fatty acid oxidation disorders or organic acidemias.Entities:
Keywords: 18-OH; AAPS; Acylcarnitine isomer; American Association of Pharmaceutical Scientists; BSA; C0; C10; C12; C14; C16; C16-OH; C16:1-OH; C18; C18:1-OH; C2; C3; C4; C4DC; C5; C5:1; C5DC; C6; C6-OH; C8; C8-OH; CE; CPDA; DP; EPI; ESI; FIA-MS/MS; Fatty acid β-oxidation disorders; HILIC; HPLC; IDA; IS; LC–MS/MS; MRM; Organic acidemia; PBS; QCs; acetylcarnitine; bovine serum albumin; butyrylcarnitine; carnitine; citrate phosphate dextrose adenine; collision energy; decanoylcarnitine; declustering potential; electrospray ion source; enhanced product ion monitoring; flow injection tandem mass spectrometry; glutarylcarnitine; hexanoylcarnitine; high performance liquid chromatography; hydrophilic interaction liquid chromatography; hydroxy hexanoylcarnitine; hydroxy octanoylcarnitine; hydroxy-hexadecanoylcarnitine; hydroxy-octadecanoylcarnitine; hydroxy-octadecenoylcarnitine; hydroxy-palmitoleoylcarnitine; information dependent acquisition; internal standard; isovalerylcarnitine; lauroylcarnitine; multiple reaction monitoring; myristoylcarnitine; octanoylcarnitine; palmitoylcarnitine; phosphate buffer solution; propionylcarnitine; quality control samples; stearoylcarnitine; succinylcarnitine and methylmalonylcarnitine; tiglylcarnitine
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Year: 2013 PMID： 23816563 DOI： 10.1016/j.jchromb.2013.05.028
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205