| Literature DB >> 23811300 |
Ken Cham-Fai Leung1, Siu-Fung Lee, Chi-Hin Wong, Chun-Pong Chak, Josie M Y Lai, Xiao-Ming Zhu, Yi-Xiang J Wang, Kathy W Y Sham, Christopher H K Cheng.
Abstract
This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.Entities:
Keywords: Cellular uptake; DMEM; Dulbecco’s modified Eagle medium; EDTA; FBS; FTIR; Fourier transform infrared; GFP; Gene delivery; MDCK; MRI; MTT; Madin–Darby Canine kidney; Magnetic resonance imaging; Magnetofection; NIB; NP; Nanoparticle; Neodymium–iron–boron; PBS; PEI; RLU; SPIO; Self-assembly; UV; ethylenediaminetetraacetic acid; fetal bovine serum; green fluorescence protein; magnetic resonance imaging; methylthiazolydiphenyl-tetrazolium bromide; nanoparticle; phosphate buffer saline; polyethylenimine; relative light unit; superparamagnetic iron oxide; ultraviolet
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Year: 2013 PMID： 23811300 DOI： 10.1016/j.ymeth.2013.06.006
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608