Hannah Blau1, Barry Linnane2, Rosemary Carzino3, Esta-Lee Tannenbaum4, Billy Skoric3, Philip J Robinson5, Colin Robertson5, Sarath C Ranganathan5. 1. Graub CF Center and Pulmonary Institute, Schneider Children's Medical Center of Israel, 14 Kaplan Street, Petah Tikva 49202, Israel; Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel. Electronic address: hblau@post.tau.ac.il. 2. Cystic Fibrosis Unit, Paediatric Department, Mid-Western Regional Hospital, Dooradoyle, Limerick, Ireland. 3. Department of Respiratory Medicine, Royal Children's Hospital, 50 Flemington Road, Parkville, Victoria 3052, Australia; Infection and Immunity Theme, Murdoch Children's Research Institute, Royal Children's Hospital, 50 Flemington Road, Parkville, Victoria 3052, Australia. 4. Department of Respiratory Medicine, Royal Children's Hospital, 50 Flemington Road, Parkville, Victoria 3052, Australia. 5. Department of Respiratory Medicine, Royal Children's Hospital, 50 Flemington Road, Parkville, Victoria 3052, Australia; Infection and Immunity Theme, Murdoch Children's Research Institute, Royal Children's Hospital, 50 Flemington Road, Parkville, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia.
Abstract
BACKGROUND: Induced sputum (IS) is feasible and safe in young CF children and is a readily accessible, non-invasive technique. However, it has not been compared to bronchoalveolar lavage (BAL), the gold standard for diagnosing lower airway infection. METHODS: We compared bacterial yield from IS and BAL in 11 non-expectorating CF children, aged 3 to 7.4 years. IS samples were obtained in 10/11 cases. RESULTS: Eight out of ten had the same predominant bacteria cultured from IS and BAL: Pseudomonas aeruginosa and Stenotrophomonas maltophilia[1], Staphylococcus aureus[3], and upper respiratory tract flora [4]. In one, Serratia marcescens and Haemophilus parainfluenzae were cultured from IS alone, whereas in one, non-group B Haemophilus influenzae was cultured from BAL alone. CONCLUSIONS: As proof of principle, IS samples showed good bacteriologic correlation with BAL. Larger studies are recommended to confirm IS as a clinically valuable tool and measure for early intervention studies in young CF children.
BACKGROUND: Induced sputum (IS) is feasible and safe in young CF children and is a readily accessible, non-invasive technique. However, it has not been compared to bronchoalveolar lavage (BAL), the gold standard for diagnosing lower airway infection. METHODS: We compared bacterial yield from IS and BAL in 11 non-expectorating CF children, aged 3 to 7.4 years. IS samples were obtained in 10/11 cases. RESULTS: Eight out of ten had the same predominant bacteria cultured from IS and BAL: Pseudomonas aeruginosa and Stenotrophomonas maltophilia[1], Staphylococcus aureus[3], and upper respiratory tract flora [4]. In one, Serratia marcescens and Haemophilus parainfluenzae were cultured from IS alone, whereas in one, non-group B Haemophilus influenzae was cultured from BAL alone. CONCLUSIONS: As proof of principle, IS samples showed good bacteriologic correlation with BAL. Larger studies are recommended to confirm IS as a clinically valuable tool and measure for early intervention studies in young CF children.
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