| Literature DB >> 23790494 |
Xiaofeng Cai1, Roberta Teta, Christoph Kohlhaas, Max Crüsemann, Reiko Ueoka, Alfonso Mangoni, Michael F Freeman, Jörn Piel.
Abstract
Hormaomycin (HRM) is a structurally remarkable peptide produced by Streptomyces griseoflavus W-384 that acts as a Streptomyces signaling metabolite and exhibits potent antibiotic activity against coryneform actinomycetes. HRM biosynthetic studies have been hampered by inconsistent and low production. To enhance fermentation titers, the role of its cluster-encoded regulatory genes was investigated. Extra copies of the putative regulators hrmA and hrmB were introduced into the wild-type strain, resulting in an increase of HRM production and its analogs up to 135-fold. For the HrmB overproducer, six bioactive analogs were isolated and characterized. This study demonstrates that HrmA and HrmB are positive regulators in HRM biosynthesis. A third gene, hrmH, was identified as encoding a protein capable of shifting the metabolic profile of HRM and its derivatives. Its manipulation resulted in the generation of an additional HRM analog.Entities:
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Year: 2013 PMID： 23790494 DOI： 10.1016/j.chembiol.2013.04.018
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521