Literature DB >> 23788323

In vivo imaging and biochemical characterization of protease function using fluorescent activity-based probes.

Laura E Edgington1, Matthew Bogyo.   

Abstract

Activity-based probes (ABPs) are reactive small molecules that covalently bind to active enzymes. When tagged with a fluorophore, ABPs serve as powerful tools to investigate enzymatic activity across a wide variety of applications. In this article, detailed protocols are provided for using fluorescent ABPs to biochemically characterize the activity of proteases in vitro. Furthermore, descriptions are provided of how these probes can be applied to image protease activity in live animals and tissues along with subsequent analysis by histology, flow cytometry, and SDS-PAGE.
© 2013 by John Wiley & Sons, Inc.

Entities:  

Keywords:  activity‐based probes; caspases; imaging; proteases

Mesh：

Substances：

Year:  2013        PMID： 23788323      PMCID： PMC3691694          DOI： 10.1002/9780470559277.ch120235

Source DB:  PubMed          Journal:  Curr Protoc Chem Biol        ISSN： 2160-4762


  20 in total

1.  Activity-based protein profiling: the serine hydrolases.

Authors:  Y Liu; M P Patricelli; B F Cravatt
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

Review 2.  Activity-based probes for the study of proteases: recent advances and developments.

Authors:  Sevnur Serim; Ute Haedke; Steven H L Verhelst
Journal:  ChemMedChem       Date:  2012-03-19       Impact factor: 3.466

3.  A fluorescent broad-spectrum proteasome inhibitor for labeling proteasomes in vitro and in vivo.

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Journal:  Chem Biol       Date:  2006-11

4.  Dynamic imaging of protease activity with fluorescently quenched activity-based probes.

Authors:  Galia Blum; Stefanie R Mullins; Kinneret Keren; Marko Fonovic; Christopher Jedeszko; Mark J Rice; Bonnie F Sloane; Matthew Bogyo
Journal:  Nat Chem Biol       Date:  2005-08-14       Impact factor: 15.040

5.  Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes.

Authors:  Galia Blum; Georges von Degenfeld; Milton J Merchant; Helen M Blau; Matthew Bogyo
Journal:  Nat Chem Biol       Date:  2007-09-09       Impact factor: 15.040

6.  An optimized activity-based probe for the study of caspase-6 activation.

Authors:  Laura E Edgington; Bram J van Raam; Martijn Verdoes; Christoph Wierschem; Guy S Salvesen; Matthew Bogyo
Journal:  Chem Biol       Date:  2012-03-23

Review 7.  New approaches for dissecting protease functions to improve probe development and drug discovery.

Authors:  Edgar Deu; Martijn Verdoes; Matthew Bogyo
Journal:  Nat Struct Mol Biol       Date:  2012-01-05       Impact factor: 15.369

8.  A nonpeptidic cathepsin S activity-based probe for noninvasive optical imaging of tumor-associated macrophages.

Authors:  Martijn Verdoes; Laura E Edgington; Ferenc A Scheeren; Melissa Leyva; Galia Blum; Kipp Weiskopf; Michael H Bachmann; Jonathan A Ellman; Matthew Bogyo
Journal:  Chem Biol       Date:  2012-05-25

9.  Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis.

Authors:  Johanna A Joyce; Amos Baruch; Kareem Chehade; Nicole Meyer-Morse; Enrico Giraudo; Fong-Ying Tsai; Doron C Greenbaum; Jeffrey H Hager; Matthew Bogyo; Douglas Hanahan
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10.  Structural and functional analysis of the interaction between the agonistic monoclonal antibody Apomab and the proapoptotic receptor DR5.

Authors:  C Adams; K Totpal; D Lawrence; S Marsters; R Pitti; S Yee; S Ross; L Deforge; H Koeppen; M Sagolla; D Compaan; H Lowman; S Hymowitz; A Ashkenazi
Journal:  Cell Death Differ       Date:  2008-01-25       Impact factor: 15.828
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  11 in total

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Authors:  Kyungmin Ji; Joshua Heyza; Dora Cavallo-Medved; Bonnie F Sloane
Journal:  Biochimie       Date:  2015-09-12       Impact factor: 4.079

2.  Detection of Active Caspases During Apoptosis Using Fluorescent Activity-Based Probes.

Authors:  Laura E Edgington-Mitchell; Matthew Bogyo
Journal:  Methods Mol Biol       Date:  2016

Review 3.  Small Molecule Active Site Directed Tools for Studying Human Caspases.

Authors:  Marcin Poreba; Aleksandra Szalek; Paulina Kasperkiewicz; Wioletta Rut; Guy S Salvesen; Marcin Drag
Journal:  Chem Rev       Date:  2015-11-09       Impact factor: 60.622

4.  DNA Origami Nanostructures Elicit Dose-Dependent Immunogenicity and Are Nontoxic up to High Doses In Vivo.

Authors:  Christopher R Lucas; Patrick D Halley; Amjad A Chowdury; Bonnie K Harrington; Larry Beaver; Rosa Lapalombella; Amy J Johnson; Erin K Hertlein; Mitch A Phelps; John C Byrd; Carlos E Castro
Journal:  Small       Date:  2022-05-28       Impact factor: 15.153

5.  Legumain is activated in macrophages during pancreatitis.

Authors:  Laura E Edgington-Mitchell; Thomas Wartmann; Alicia K Fleming; Vasilena Gocheva; Wouter A van der Linden; Nimali P Withana; Martijn Verdoes; Luigi Aurelio; Daniel Edgington-Mitchell; TinaMarie Lieu; Belinda S Parker; Bim Graham; Thomas Reinheckel; John B Furness; Johanna A Joyce; Peter Storz; Walter Halangk; Matthew Bogyo; Nigel W Bunnett
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2016-08-11       Impact factor: 4.052

6.  Application of a Sulfoxonium Ylide Electrophile to Generate Cathepsin X-Selective Activity-Based Probes.

Authors:  Simon J Mountford; Bethany M Anderson; Bangyan Xu; Elean S V Tay; Monika Szabo; My-Linh Hoang; Jiayin Diao; Luigi Aurelio; Rhiannon I Campden; Erik Lindström; Erica K Sloan; Robin M Yates; Nigel W Bunnett; Philip E Thompson; Laura E Edgington-Mitchell
Journal:  ACS Chem Biol       Date:  2020-02-14       Impact factor: 5.100

7.  System-wide biochemical analysis reveals ozonide antimalarials initially act by disrupting Plasmodium falciparum haemoglobin digestion.

Authors:  Carlo Giannangelo; Ghizal Siddiqui; Amanda De Paoli; Bethany M Anderson; Laura E Edgington-Mitchell; Susan A Charman; Darren J Creek
Journal:  PLoS Pathog       Date:  2020-06-26       Impact factor: 6.823

8.  Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer.

Authors:  Laura E Edgington-Mitchell; Jai Rautela; Hendrika M Duivenvoorden; Krishnath M Jayatilleke; Wouter A van der Linden; Martijn Verdoes; Matthew Bogyo; Belinda S Parker
Journal:  Oncotarget       Date:  2015-09-29

Review 9.  Optical imaging probes in oncology.

Authors:  Cristina Martelli; Alessia Lo Dico; Cecilia Diceglie; Giovanni Lucignani; Luisa Ottobrini
Journal:  Oncotarget       Date:  2016-07-26

10.  Application of a chemical probe to detect neutrophil elastase activation during inflammatory bowel disease.

Authors:  Bethany M Anderson; Daniel P Poole; Luigi Aurelio; Garrett Z Ng; Markus Fleischmann; Paulina Kasperkiewicz; Celine Morissette; Marcin Drag; Ian R van Driel; Brian L Schmidt; Stephen J Vanner; Nigel W Bunnett; Laura E Edgington-Mitchell
Journal:  Sci Rep       Date:  2019-09-16       Impact factor: 4.379
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