| Literature DB >> 23776209 |
Feng Wang1, Dhinakaran Sambandan, Rajkumar Halder, Jianing Wang, Sarah M Batt, Brian Weinrick, Insha Ahmad, Pengyu Yang, Yong Zhang, John Kim, Morad Hassani, Stanislav Huszar, Claudia Trefzer, Zhenkun Ma, Takushi Kaneko, Khisi E Mdluli, Scott Franzblau, Arnab K Chatterjee, Kai Johnsson, Kai Johnson, Katarina Mikusova, Gurdyal S Besra, Klaus Fütterer, Scott H Robbins, S Whitney Barnes, John R Walker, William R Jacobs, Peter G Schultz.
Abstract
A cell-based phenotypic screen for inhibitors of biofilm formation in mycobacteria identified the small molecule TCA1, which has bactericidal activity against both drug-susceptible and -resistant Mycobacterium tuberculosis (Mtb) and sterilizes Mtb in vitro combined with rifampicin or isoniazid. In addition, TCA1 has bactericidal activity against nonreplicating Mtb in vitro and is efficacious in acute and chronic Mtb infection mouse models both alone and combined with rifampicin or isoniazid. Transcriptional analysis revealed that TCA1 down-regulates genes known to be involved in Mtb persistence. Genetic and affinity-based methods identified decaprenyl-phosphoryl-β-D-ribofuranose oxidoreductase DprE1 and MoeW, enzymes involved in cell wall and molybdenum cofactor biosynthesis, respectively, as targets responsible for the activity of TCA1. These in vitro and in vivo results indicate that this compound functions by a unique mechanism and suggest that TCA1 may lead to the development of a class of antituberculosis agents.Entities:
Keywords: drug resistance; dual mechanism
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Year: 2013 PMID： 23776209 PMCID： PMC3703973 DOI： 10.1073/pnas.1309171110
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205