OBJECTIVE: Cartilage repair of full-thickness chondral defects in the knees of Goettinger minipigs was assessed after treatment with cell-free collagen type-I gel with or without additional BMP-7 loaded poly(lactic-co-glycolid acid) microspheres. METHODS: Two full-thickness chondral defects were created in the trochlear groove of one hind leg knee in six Goettinger minipigs. Six defects were treated with a cell-free collagen type-I gel plug of 10 mm, the corresponding six defects were treated with a cell-free collagen type-I plug with poly(lactic-co-glycolid acid) microspheres loaded with recombinant BMP-7 (100 ng/ml gel). After 1 year, the animals were sacrificed. Immediately after recovery, non-destructive biomechanical testing was performed. The repair tissue quality was evaluated by immunohistochemistry and the O'Driscoll score was calculated. RESULTS: After 1 year, a robust cellular migration into the cell-free collagen gel plugs occurred and a hyaline-like repair tissue was found. Collagen type-II production and cellular organisation were higher in the BMP-7 microsphere group. The determination of the E-modulus, creep and relaxation revealed that mechanical properties of the BMP-7 microsphere group in summary were closer to control hyaline cartilage. CONCLUSIONS: While all specimens revealed a homogeneous cellular distribution, ECM production, cellular organisation and mechanical properties were enhanced by continuous BMP-7 release.
OBJECTIVE:Cartilage repair of full-thickness chondral defects in the knees of Goettinger minipigs was assessed after treatment with cell-free collagen type-I gel with or without additional BMP-7 loaded poly(lactic-co-glycolid acid) microspheres. METHODS: Two full-thickness chondral defects were created in the trochlear groove of one hind leg knee in six Goettinger minipigs. Six defects were treated with a cell-free collagen type-I gel plug of 10 mm, the corresponding six defects were treated with a cell-free collagen type-I plug with poly(lactic-co-glycolid acid) microspheres loaded with recombinant BMP-7 (100 ng/ml gel). After 1 year, the animals were sacrificed. Immediately after recovery, non-destructive biomechanical testing was performed. The repair tissue quality was evaluated by immunohistochemistry and the O'Driscoll score was calculated. RESULTS: After 1 year, a robust cellular migration into the cell-free collagen gel plugs occurred and a hyaline-like repair tissue was found. Collagen type-II production and cellular organisation were higher in the BMP-7 microsphere group. The determination of the E-modulus, creep and relaxation revealed that mechanical properties of the BMP-7 microsphere group in summary were closer to control hyaline cartilage. CONCLUSIONS: While all specimens revealed a homogeneous cellular distribution, ECM production, cellular organisation and mechanical properties were enhanced by continuous BMP-7 release.
Authors: Victoria Horbert; Long Xin; Peter Foehr; Olaf Brinkmann; Matthias Bungartz; Rainer H Burgkart; T Graeve; Raimund W Kinne Journal: Cartilage Date: 2018-02-20 Impact factor: 4.634
Authors: Guojing Sun; Jianmin Chen; Shufeng Yang; Thomas Mn Parker; Gary Mp Goodman; Jack M Hasama; Jianning Zhao Journal: Int J Clin Exp Med Date: 2015-12-15
Authors: Gregor Reisig; Michael Kreinest; Wiltrud Richter; Mechthild Wagner-Ecker; Dietmar Dinter; Ulrike Attenberger; Barbara Schneider-Wald; Stefan Fickert; Markus L Schwarz Journal: PLoS One Date: 2016-11-07 Impact factor: 3.240