| Literature DB >> 23770096 |
Linda Sundström1, Peter J Greasley, Susanna Engberg, Malin Wallander, Erik Ryberg.
Abstract
Succinate has been reported as the endogenous ligand for GPR91. In this study, succinate was confirmed to activate GPR91 resulting in both 3'-5'-cyclic adenosine monophosphate (cAMP) inhibition and inositol phosphate formation in a pertussis toxin (PTX)-sensitive manner. GPR91 agonist-mediated effects detected using dynamic mass redistribution (DMR) were inhibited with PTX, edelfosine and U73122 demonstrating the importance of not only the Gαi pathway but also PLCβ. These results show that GPR91 when expressed in HEK293s cells couples exclusively through the Gαi pathway and acts through Gαi not only to inhibit cAMP production but also to increase intracellular Ca(2+) in an inositol phosphate dependent mechanism via PLCβ activation.Entities:
Keywords: 3′–5′-cyclic adenosine monophosphate; BSA; DMR; ERK; FACS; FLIPR; GPCR; GPR91; GTPγS; HTRF; IP(1); MAPK; PLCβ; PTX; SUCNR1; Succinate; bovine serum albumin; cAMP; dynamic mass redistribution; extracellular-signal-regulated kinase; fluorescence-activated cell sorting; fluorescent imaging plate reader; guanosine 5′-[γ-(35)S]-triphosphate; homogeneous time resolved fluorescence; inositol-1-phosphate; mitogen-activated protein kinase; pertussis toxin; succinate receptor 1
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Year: 2013 PMID： 23770096 DOI： 10.1016/j.febslet.2013.05.067
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124