| Literature DB >> 23767758 |
Pu Guo1, Shengyong Yan, Jianlin Hu, Xiwen Xing, Changcheng Wang, Xiaowei Xu, Xiaoyu Qiu, Wen Ma, Chunjiang Lu, Xiaocheng Weng, Xiang Zhou.
Abstract
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.Entities:
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Year: 2013 PMID： 23767758 DOI： 10.1021/ol401290d
Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005