Literature DB >> 23767

The active site of beta-glucosidase from Botryodiplodia theobromae. Effects of pH and dioxan on enzyme-catalysed reactions.

G M Umezurike.   

Abstract

1. The hydrolysis of o-nitrophenyl beta-D-glucopyranoside by the high-molecular-weight beta-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) of Botryodiplodia theobromae Pat in the absence or presence of added dioxan was found to be dependent on the ionization of two groups, which appeared to be a carboxyl group and an imidazole group. 2. Dioxan increased the Michaelis constant, Km, but decreased the maximum velocity, V.

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Year:  1977        PMID： 23767      PMCID： PMC1183732          DOI： 10.1042/bj1670831

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  The subunit structure of beta-glucosidase from Botryodiplodia theobromae Pat.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

2.  Kinetic analysis of the mechanism of action of beta-glucosidase from Botryodiplodia theobromae Pat.

Authors:  G M Umezurike
Journal:  Biochim Biophys Acta       Date:  1975-07-27

3.  PEPTIDES DERIVED FROM TRYPTIC DIGESTION OF EGG WHITE LYSOZYME.

Authors:  R E CANFIELD
Journal:  J Biol Chem       Date:  1963-08       Impact factor: 5.157

4.  The mechanism of ficin-catalysed reactions.

Authors:  B R HAMMOND; H GUTFREUND
Journal:  Biochem J       Date:  1959-06       Impact factor: 3.857

5.  The active site and mechanism of action of bovine pancreatic ribonuclease. 5. The charge types at the active centre.

Authors:  D Findlay; A P Mathias; B R Rabin
Journal:  Biochem J       Date:  1962-10       Impact factor: 3.857

6.  Kinetic properties of -glucosidase from Botryodiplodia theobromae Pat.

Authors:  G M Umezurike
Journal:  Biochim Biophys Acta       Date:  1971-10

7.  Inhibition, transgalactosylation and mechanism of action of sweet almond alpha-galactosidase.

Authors:  P M Dey
Journal:  Biochim Biophys Acta       Date:  1969

8.  The purification and properties of extracellular beta-glucosidase from Botryodiplodia theobromae Pat.

Authors:  G M Umezurike
Journal:  Biochim Biophys Acta       Date:  1971-02-10

9.  Substrate specificity and kinetic properties of alpha-galactosidases from Vicia faba.

Authors:  P M Dey; J B Pridham
Journal:  Biochem J       Date:  1969-10       Impact factor: 3.857

10.  The effect of methanol and dioxan on the rates of the beta-galactosidase-catalysed hydrolyses of some beta-D-galactrophyranosides: rate-limiting degalactosylation. The ph-dependence of galactosylation and degalactosylation.

Authors:  M L Sinnott; O M Viratelle
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857
  10 in total
  5 in total

1.  Chemical mechanism of beta-glucosidase from Trichoderma reesei QM 9414. pH-dependence of kinetic parameters.

Authors:  I de la Mata; P Estrada; R Macarrón; J M Dominguez; M P Castillón; C Acebal
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

2.  The beta-glucosidase from Botryodiplodia theobromae. Mechanism of enzyme-catalysed reactions.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1979-06-01       Impact factor: 3.857

3.  The beta-glucosidase from Botryodiplodia theobromae Pat. Kinetics of enzyme-catalysed hydrolysis of o-nitrophenyl beta-D-glucopyranoside in dioxan/water.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857

4.  The mechanism of action of beta-glucosidase from Botryodiplodia theobromae Pat.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1987-01-15       Impact factor: 3.857

5.  The beta-glucosidase from Botryodiplodia theobromae. Mechanism of enzyme action.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857
  5 in total

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