Marcos Henrique Barreta1, Bernardo Garziera Gasperin2, Rogério Ferreira3, Monique Rovani2, Gabriel Ribas Pereira2, Rodrigo C Bohrer4, João Francisco de Oliveira2, Paulo Bayard Dias Gonçalves2. 1. Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil Universidade Federal de Santa Catarina, Campus Universitário de Curitibanos, Brazil marcos.barreta@ufsc.br. 2. Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil. 3. Centro de Educação Superior do Oeste-Universidade do Estado de Santa Catarina, Brasil. 4. Laboratório de Biotecnologia e Reprodução Animal-BioRep, Universidade Federal de Santa Maria, Brazil Department of Animal Science, McGill University, Ste-Anne-De-Bellevue, QC, Canada.
Abstract
INTRODUCTION: This study was based on the hypothesis that some components of the angiotensin-(1-7) (Ang-(1-7)) system are differentially expressed during follicular development and can be involved in the follicular health/atresia transition in bovine. MATERIAL AND METHODS: The largest (F1) and second largest follicles (F2) were collected from cows before (Day 2), during (Day 3), or after (Day 4) the expected moment of follicular deviation. In the second experiment, F1 was induced to atresia through intrafollicular injection of fulvestrant (estrogen receptor-antagonist) and, in both experiments, mRNA expression of the Mas receptor, ACE2, NEP, and PEP was evaluated in the granulosa and theca cells. RESULTS: The mRNA expression of Mas receptor was upregulated in the granulosa cells of F2 after the establishment of follicular deviation, while PEP mRNA increased during and after the deviation process. The mRNA expression of ACE2 was upregulated in the granulosa cells of F1 during and after the follicular deviation. The mRNA expression of NEP was not regulated in F1 and F2. Mas receptor expression increased in the F1 induced to atresia. CONCLUSIONS: mRNA for Mas receptor, ACE2, and PEP are differentially expressed in granulosa cells throughout follicular development and the Mas receptor can be involved with the establishment of follicular dominance.
INTRODUCTION: This study was based on the hypothesis that some components of the angiotensin-(1-7) (Ang-(1-7)) system are differentially expressed during follicular development and can be involved in the follicular health/atresia transition in bovine. MATERIAL AND METHODS: The largest (F1) and second largest follicles (F2) were collected from cows before (Day 2), during (Day 3), or after (Day 4) the expected moment of follicular deviation. In the second experiment, F1 was induced to atresia through intrafollicular injection of fulvestrant (estrogen receptor-antagonist) and, in both experiments, mRNA expression of the Mas receptor, ACE2, NEP, and PEP was evaluated in the granulosa and theca cells. RESULTS: The mRNA expression of Mas receptor was upregulated in the granulosa cells of F2 after the establishment of follicular deviation, while PEP mRNA increased during and after the deviation process. The mRNA expression of ACE2 was upregulated in the granulosa cells of F1 during and after the follicular deviation. The mRNA expression of NEP was not regulated in F1 and F2. Mas receptor expression increased in the F1 induced to atresia. CONCLUSIONS: mRNA for Mas receptor, ACE2, and PEP are differentially expressed in granulosa cells throughout follicular development and the Mas receptor can be involved with the establishment of follicular dominance.