Literature DB >> 23756466

Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases.

Yael Michaeli1, Tamar Shahal, Dmitry Torchinsky, Assaf Grunwald, Rony Hoch, Yuval Ebenstein.   

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

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Year:  2013        PMID： 23756466     DOI： 10.1039/c3cc42543f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  21 in total

1.  Visualizing the entire DNA from a chromosome in a single frame.

Authors:  C Freitag; C Noble; J Fritzsche; F Persson; M Reiter-Schad; A N Nilsson; A Granéli; T Ambjörnsson; K U Mir; J O Tegenfeldt
Journal:  Biomicrofluidics       Date:  2015-08-05       Impact factor: 2.800

Review 2.  Advances in the profiling of DNA modifications: cytosine methylation and beyond.

Authors:  Nongluk Plongthongkum; Dinh H Diep; Kun Zhang
Journal:  Nat Rev Genet       Date:  2014-08-27       Impact factor: 53.242

3.  Global modulation in DNA epigenetics during pro-inflammatory macrophage activation.

Authors:  Nikhil Jain; Tamar Shahal; Tslil Gabrieli; Noa Gilat; Dmitry Torchinsky; Yael Michaeli; Viola Vogel; Yuval Ebenstein
Journal:  Epigenetics       Date:  2019-07-08       Impact factor: 4.528

4.  Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.

Authors:  Dmitry Torchinsky; Yael Michaeli; Natalie R Gassman; Yuval Ebenstein
Journal:  Chem Commun (Camb)       Date:  2019-09-19       Impact factor: 6.222

5.  Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation.

Authors:  Chun-Xiao Song; Jiajie Diao; Axel T Brunger; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2016-03-28       Impact factor: 11.205

Review 6.  Analytical epigenetics: single-molecule optical detection of DNA and histone modifications.

Authors:  Christian Heck; Yael Michaeli; Ilko Bald; Yuval Ebenstein
Journal:  Curr Opin Biotechnol       Date:  2018-10-13       Impact factor: 9.740

7.  Bacteriophage strain typing by rapid single molecule analysis.

Authors:  Assaf Grunwald; Moran Dahan; Anna Giesbertz; Adam Nilsson; Lena K Nyberg; Elmar Weinhold; Tobias Ambjörnsson; Fredrik Westerlund; Yuval Ebenstein
Journal:  Nucleic Acids Res       Date:  2015-05-27       Impact factor: 16.971

Review 8.  Hydroxymethylation of DNA: an epigenetic marker.

Authors:  Rajneesh Richa; Rajeshwar P Sinha
Journal:  EXCLI J       Date:  2014-05-27       Impact factor: 4.068

9.  Sizing femtogram amounts of dsDNA by single-molecule counting.

Authors:  Dmitry Torchinsky; Yuval Ebenstein
Journal:  Nucleic Acids Res       Date:  2015-09-13       Impact factor: 16.971

10.  Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers.

Authors:  Noa Gilat; Tzlil Tabachnik; Amit Shwartz; Tamar Shahal; Dmitry Torchinsky; Yael Michaeli; Gil Nifker; Shahar Zirkin; Yuval Ebenstein
Journal:  Clin Epigenetics       Date:  2017-07-14       Impact factor: 6.551
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