Literature DB >> 23750483

Production of a monoclonal antibody specific for Pou5f1/Oct4.

Tatsuhiko Arakawa1, Tomohiko Yoshimi, Masayuki Azuma, Taro Tachibana.   

Abstract

Pou5f1/Oct4, a member of the POU transcription factor family, is exclusively expressed in embryonic stem cells, which are involved in self-renewal and maintaining pluripotency. In the present study, we report on the establishment of a monoclonal antibody that is specific for Oct4 using the rat medial iliac lymph node method. In an immunoblotting analysis, our antibody detected endogenous Oct4. In addition, immunocytochemical staining using the antibody revealed the nuclear localization of Oct4. This monoclonal antibody has the potential for use in the further analysis of Oct4 function in stem cells.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23750483      PMCID： PMC3732135          DOI： 10.1089/mab.2013.0004

Source DB:  PubMed          Journal:  Monoclon Antib Immunodiagn Immunother        ISSN： 2167-9436


  13 in total

Review 1.  Molecular mechanism to maintain stem cell renewal of ES cells.

Authors:  H Niwa
Journal:  Cell Struct Funct       Date:  2001-06       Impact factor: 2.212

2.  Production of a rat monoclonal antibody specific for Myf5.

Authors:  Akihito Harada; Seiji Okada; Jun Odawara; Hiromi Kumamaru; Hirokazu Saiwai; Mayumi Aoki; Mako Nakamura; Yuko Nishiyama; Yasuyuki Ohkawa
Journal:  Hybridoma (Larchmt)       Date:  2010-02

3.  Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different alpha chains of human type IV collagen.

Authors:  Y Sado; M Kagawa; Y Kishiro; K Sugihara; I Naito; J M Seyer; M Sugimoto; T Oohashi; Y Ninomiya
Journal:  Histochem Cell Biol       Date:  1995-10       Impact factor: 4.304

4.  Production of a rat monoclonal antibody against Brg1.

Authors:  Yasuyuki Ohkawa; Akihito Harada; Mako Nakamura; Saori Yoshimura; Taro Tachibana
Journal:  Hybridoma (Larchmt)       Date:  2009-12

5.  New type of POU domain in germ line-specific protein Oct-4.

Authors:  H R Schöler; S Ruppert; N Suzuki; K Chowdhury; P Gruss
Journal:  Nature       Date:  1990-03-29       Impact factor: 49.962

Review 6.  Oct-4: control of totipotency and germline determination.

Authors:  M Pesce; H R Schöler
Journal:  Mol Reprod Dev       Date:  2000-04       Impact factor: 2.609

7.  A novel method of preparing rat-monoclonal antibody-producing hybridomas by using rat medial iliac lymph node cells.

Authors:  Y Kishiro; M Kagawa; I Naito; Y Sado
Journal:  Cell Struct Funct       Date:  1995-04       Impact factor: 2.212

Review 8.  Octamania: the POU factors in murine development.

Authors:  H R Schöler
Journal:  Trends Genet       Date:  1991-10       Impact factor: 11.639

9.  Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Authors:  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka
Journal:  Cell       Date:  2007-11-30       Impact factor: 41.582

10.  Cooperative activation of muscle gene expression by MEF2 and myogenic bHLH proteins.

Authors:  J D Molkentin; B L Black; J F Martin; E N Olson
Journal:  Cell       Date:  1995-12-29       Impact factor: 41.582
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.