Literature DB >> 20025509

Production of a rat monoclonal antibody against Brg1.

Yasuyuki Ohkawa1, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana.   

Abstract

Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

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Year:  2009        PMID： 20025509     DOI： 10.1089/hyb.2009.0041

Source DB:  PubMed          Journal:  Hybridoma (Larchmt)        ISSN： 1554-0014


  6 in total

1.  Production of a monoclonal antibody specific for Pou5f1/Oct4.

Authors:  Tatsuhiko Arakawa; Tomohiko Yoshimi; Masayuki Azuma; Taro Tachibana
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2013-06

2.  Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2.

Authors:  Masako Tanaka; Maki Yamaguchi; Masayuki Shiota; Yukiko Kawamoto; Katsuyuki Takahashi; Azusa Inagaki; Mayuko Osada-Oka; Akihito Harada; Hideki Wanibuchi; Yasukatsu Izumi; Katsuyuki Miura; Hiroshi Iwao; Yasuyuki Ohkawa
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2014-08

3.  Chd2 interacts with H3.3 to determine myogenic cell fate.

Authors:  Akihito Harada; Seiji Okada; Daijiro Konno; Jun Odawara; Tomohiko Yoshimi; Saori Yoshimura; Hiromi Kumamaru; Hirokazu Saiwai; Toshiaki Tsubota; Hitoshi Kurumizaka; Koichi Akashi; Taro Tachibana; Anthony N Imbalzano; Yasuyuki Ohkawa
Journal:  EMBO J       Date:  2012-05-08       Impact factor: 11.598

4.  Spatial re-organization of myogenic regulatory sequences temporally controls gene expression.

Authors:  Akihito Harada; Chandrashekara Mallappa; Seiji Okada; John T Butler; Stephen P Baker; Jeanne B Lawrence; Yasuyuki Ohkawa; Anthony N Imbalzano
Journal:  Nucleic Acids Res       Date:  2015-02-04       Impact factor: 16.971

5.  Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas.

Authors:  Yuki Kuniyoshi; Kazumitsu Maehara; Takeshi Iwasaki; Masayasu Hayashi; Yuichiro Semba; Masatoshi Fujita; Yuko Sato; Hiroshi Kimura; Akihito Harada; Yasuyuki Ohkawa
Journal:  PLoS One       Date:  2016-10-27       Impact factor: 3.240

6.  Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells.

Authors:  Yuichiro Semba; Akihito Harada; Kazumitsu Maehara; Shinya Oki; Chikara Meno; Jun Ueda; Kazuo Yamagata; Atsushi Suzuki; Mitsuho Onimaru; Jumpei Nogami; Seiji Okada; Koichi Akashi; Yasuyuki Ohkawa
Journal:  Nucleic Acids Res       Date:  2017-09-06       Impact factor: 16.971
  6 in total

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