Literature DB >> 2374717

Constructing DNA by polymerase recombination.

A A Yolov1, Z A Shabarova.   

Abstract

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to be sufficient to provide further DNA joining also by use of PCR.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2374717      PMCID： PMC331102          DOI： 10.1093/nar/18.13.3983

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  [Construction and expression of genes encoding short hybrid mRNA in Escherichia coli].

Authors:  E A Skripkin; S L Bogdanova; A M Kopylov; A A Bogdanov
Journal:  Dokl Akad Nauk SSSR       Date:  1986 Mar-Apr

2.  A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

Authors:  R Higuchi; B Krummel; R K Saiki
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

3.  Genomic amplification with transcript sequencing.

Authors:  E S Stoflet; D D Koeberl; G Sarkar; S S Sommer
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

4.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

5.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

Authors:  R M Horton; H D Hunt; S N Ho; J K Pullen; L R Pease
Journal:  Gene       Date:  1989-04-15       Impact factor: 3.688

6.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

7.  [Automated synthesis of oligodeoxyribonucleotides with terminal phosphate groups].

Authors:  E M Volkov; E A Romanova; A Krug; T S Oretskaia; V K Potapov
Journal:  Bioorg Khim       Date:  1988-08
  7 in total
  15 in total

1.  Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously.

Authors:  Nikolai A Shevchuk; Anton V Bryksin; Yevgeniya A Nusinovich; Felipe C Cabello; Margaret Sutherland; Stephan Ladisch
Journal:  Nucleic Acids Res       Date:  2004-01-22       Impact factor: 16.971

2.  Mechanisms of intermolecular homologous recombination in plants as studied with single- and double-stranded DNA molecules.

Authors:  M J de Groot; R Offringa; M P Does; P J Hooykaas; P J van den Elzen
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

3.  A method for construction, cloning and expression of intron-less gene from unannotated genomic DNA.

Authors:  Vineet Agrawal; Bharti Gupta; Uttam Chand Banerjee; Nilanjan Roy
Journal:  Mol Biotechnol       Date:  2008-06-10       Impact factor: 2.695

4.  Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA.

Authors:  Leslie A Mitchell; Yizhi Cai; Martin Taylor; Anne Marie Noronha; James Chuang; Lixin Dai; Jef D Boeke
Journal:  ACS Synth Biol       Date:  2013-03-11       Impact factor: 5.110

5.  Conformational heterogeneity of the SAM-I riboswitch transcriptional ON state: a chaperone-like role for S-adenosyl methionine.

Authors:  Wei Huang; Joohyun Kim; Shantenu Jha; Fareed Aboul-Ela
Journal:  J Mol Biol       Date:  2012-03-13       Impact factor: 5.469

6.  PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes.

Authors:  R M Horton
Journal:  Mol Biotechnol       Date:  1995-04       Impact factor: 2.695

7.  Oligodendroglial and pan-neural crest expression of Cre recombinase directed by Sox10 enhancer.

Authors:  Zachary E Stine; Jimmy L Huynh; Stacie K Loftus; David U Gorkin; Amirali H Salmasi; Thomas Novak; Todd Purves; Ronald A Miller; Anthony Antonellis; John P Gearhart; William J Pavan; Andrew S McCallion
Journal:  Genesis       Date:  2009-11       Impact factor: 2.487

8.  Conversion of 3-chlorocatechol by various catechol 2,3-dioxygenases and sequence analysis of the chlorocatechol dioxygenase region of Pseudomonas putida GJ31.

Authors:  A E Mars; J Kingma; S R Kaschabek; W Reineke; D B Janssen
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

9.  Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones.

Authors:  B R Krishnan; D Kersulyte; I Brikun; C M Berg; D E Berg
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

10.  Quantitative PCR as a method for monitoring retroviral infection on the gene level.

Authors:  A A Yolov; A V Kozlova; N G Yaroslavtseva; B M Mednikov; E V Karamov
Journal:  Virus Genes       Date:  1995       Impact factor: 2.332
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.