Literature DB >> 2374710

An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis.

M J Damha1, P A Giannaris, S V Zabarylo.   

Abstract

A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.

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Year:  1990        PMID： 2374710      PMCID： PMC331081          DOI： 10.1093/nar/18.13.3813

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  2 in total

1.  Derivatization of controlled pore glass beads for solid phase oligonucleotide synthesis.

Authors:  R T Pon; N Usman; K K Ogilvie
Journal:  Biotechniques       Date:  1988-09       Impact factor: 1.993

2.  Total chemical synthesis of a 77-nucleotide-long RNA sequence having methionine-acceptance activity.

Authors:  K K Ogilvie; N Usman; K Nicoghosian; R J Cedergren
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205
  2 in total
  24 in total

1.  2'-Deoxy-2'-fluoro-beta-D-arabinonucleosides and oligonucleotides (2'F-ANA): synthesis and physicochemical studies.

Authors:  C J Wilds; M J Damha
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

2.  Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.

Authors:  R T Pon; S Yu
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

3.  Molecular requirements for degradation of a modified sense RNA strand by Escherichia coli ribonuclease H1.

Authors:  Daniel R Yazbeck; Kyung-Lyum Min; Masad J Damha
Journal:  Nucleic Acids Res       Date:  2002-07-15       Impact factor: 16.971

4.  One pot general method for the derivatisation of polymer support for oligonucleotide synthesis.

Authors:  P Sharma; A K Sharma; V P Malhotra; K C Gupta
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

5.  RAPID FUNCTIONALIZATION AND LOADING OF SOLID SUPPORTS.

Authors:  Radhakrishnan P Iyer; John E Coughlin; Seetharamaiyer Padmanabhan
Journal:  Org Prep Proced Int       Date:  2005-06       Impact factor: 1.628

6.  Synthesis of native-like crosslinked duplex RNA and study of its properties.

Authors:  Kazumitsu Onizuka; Madoka E Hazemi; Justin M Thomas; Leanna R Monteleone; Ken Yamada; Shuhei Imoto; Peter A Beal; Fumi Nagatsugi
Journal:  Bioorg Med Chem       Date:  2017-02-21       Impact factor: 3.641

7.  4'-Thio-oligo-beta-D-ribonucleotides: synthesis of beta-4'-thio-oligouridylates, nuclease resistance, base pairing properties, and interaction with HIV-1 reverse transcriptase.

Authors:  L Bellon; J L Barascut; G Maury; G Divita; R Goody; J L Imbach
Journal:  Nucleic Acids Res       Date:  1993-04-11       Impact factor: 16.971

8.  Characterization of fully 2'-modified oligoribonucleotide hetero- and homoduplex hybridization and nuclease sensitivity.

Authors:  L L Cummins; S R Owens; L M Risen; E A Lesnik; S M Freier; D McGee; C J Guinosso; P D Cook
Journal:  Nucleic Acids Res       Date:  1995-06-11       Impact factor: 16.971

9.  Oligoribonucleotides containing 2',5'-phosphodiester linkages exhibit binding selectivity for 3',5'-RNA over 3',5'-ssDNA.

Authors:  P A Giannaris; M J Damha
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

10.  Solid-phase synthesis and hybridization properties of DNA containing sulfide-linked dinucleosides.

Authors:  S H Kawai; D Wang; P A Giannaris; M J Damha; G Just
Journal:  Nucleic Acids Res       Date:  1993-03-25       Impact factor: 16.971
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