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13 in total

1. DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Authors: Alexandra Heininger; Marlies Binder; Andreas Ellinger; Konrad Botzenhart; Klaus Unertl; Gerd Döring
Journal: J Clin Microbiol Date: 2003-04 Impact factor: 5.948

Review 2. Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

Authors: Geraint B Rogers; Kenneth D Bruce
Journal: Mol Diagn Ther Date: 2010-12-01 Impact factor: 4.074

3. Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Authors: Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Andreas Hoeft; Frank Stuber
Journal: Mol Biotechnol Date: 2002-11 Impact factor: 2.695

Review 4. False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.

Authors: A Borst; A T A Box; A C Fluit
Journal: Eur J Clin Microbiol Infect Dis Date: 2004-03-10 Impact factor: 3.267

10. Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.

Authors: Rudolph Spangler; Noel L Goddard; David S Thaler
Journal: PLoS One Date: 2009-09-15 Impact factor: 3.240

Improving PCR efficiency.

1. DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Review 2. Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

3. Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Review 4. False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.

5. Use of psoralen as extinguisher of contaminated DNA in PCR.

6. Use of gamma irradiation to eliminate DNA contamination for PCR.

7. The use of exonuclease III for polymerase chain reaction sterilization.

8. RS46(DXS548) genotyping of reproductive cells: approaching preimplantation testing of the fragile-X syndrome.

Review 9. Inhibition and facilitation of nucleic acid amplification.

10. Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.