Literature DB >> 2374548

Purification and characterization of L-dopa decarboxylase from human kidney.

D G Mappouras1, J Stiakakis, E G Fragoulis.   

Abstract

L-dopa decarboxylase has been purified to homogeneity from post mortem removed human kidneys. Homogeneity was examined by polyacrylamide gel electrophoresis (PAGE) analysis both in the presence and absence of SDS. The enzyme has a molecular weight of 100,000 daltons estimated by gel filtration and 50,000 daltons determined after SDS-PAGE. Human L-dopa decarboxylase therefore is a dimer. Polyclonal antibodies produced against human L-dopa decarboxylase react with the 50,000 daltons enzyme subunit after immuno-blotting and also precipitates enzyme activity. Activity against L-dopa is partially inhibited by 5-hydroxytryptophan (5-HTP). The effect of various cations on L-dopa decarboxylase activity has also been tested.

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Year:  1990        PMID： 2374548     DOI： 10.1007/bf00214121

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  22 in total

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Authors:  J G Christenson; W Dairman; S Udenfriend
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  14 in total

1.  L-Dopa decarboxylase expression profile in human cancer cells.

Authors:  Ioanna Chalatsa; Eleftheria Nikolouzou; Emmanuel G Fragoulis; Dido Vassilacopoulou
Journal:  Mol Biol Rep       Date:  2010-06-11       Impact factor: 2.316

2.  Purification of an endogenous inhibitor of L-Dopa decarboxylase activity from human serum.

Authors:  Alice-Georgia Vassiliou; Dido Vassilacopoulou; Emmanuel G Fragoulis
Journal:  Neurochem Res       Date:  2005-05       Impact factor: 3.996

3.  Release of membrane-associated L-dopa decarboxylase from human cells.

Authors:  Ioanna Chalatsa; Emmanuel G Fragoulis; Dido Vassilacopoulou
Journal:  Neurochem Res       Date:  2011-04-11       Impact factor: 3.996

4.  l-DOPA Decarboxylase (DDC) Expression Status as a Novel Molecular Tumor Marker for Diagnostic and Prognostic Purposes in Laryngeal Cancer.

Authors:  Christos Patsis; Vasiliki Glyka; Ioannis Yiotakis; Emmanuel G Fragoulis; Andreas Scorilas
Journal:  Transl Oncol       Date:  2012-08-01       Impact factor: 4.243

5.  Inhibition of aromatic L-amino acid decarboxylase activity by human autoantibodies.

Authors:  E S Husebye; A S Boe; F Rorsman; O Kämpe; A Aakvaag; T Rygh; T Flatmark; J Haavik
Journal:  Clin Exp Immunol       Date:  2000-06       Impact factor: 4.330

6.  L-DOPA decarboxylase association with membranes in mouse brain.

Authors:  P Poulikakos; D Vassilacopoulou; E G Fragoulis
Journal:  Neurochem Res       Date:  2001-05       Impact factor: 3.996

7.  Identification and characterization of a novel form of the human L-dopa decarboxylase mRNA.

Authors:  Dido Vassilacopoulou; Diamantis C Sideris; Alice G Vassiliou; Emmanuel G Fragoulis
Journal:  Neurochem Res       Date:  2004-10       Impact factor: 3.996

8.  Cloning and expression of human placental L-Dopa decarboxylase.

Authors:  Maria-Zaharenia Siaterli; Dido Vassilacopoulou; Emmanuel G Fragoulis
Journal:  Neurochem Res       Date:  2003-06       Impact factor: 3.996

9.  L-Dopa decarboxylase (DDC) constitutes an emerging biomarker in predicting patients' survival with stomach adenocarcinomas.

Authors:  Dimitra Florou; Iordanis N Papadopoulos; Emmanuel G Fragoulis; Andreas Scorilas
Journal:  J Cancer Res Clin Oncol       Date:  2012-10-12       Impact factor: 4.553

10.  Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma.

Authors:  C K Kontos; I N Papadopoulos; E G Fragoulis; A Scorilas
Journal:  Br J Cancer       Date:  2010-04-27       Impact factor: 7.640
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