Milind M Muley1, Vishnu N Thakare2, Rajesh R Patil2, Pallavi A Bafna1, Suresh R Naik3. 1. Department of Pharmacology, Padm. Dr. D.Y. Patil Institute of Pharmaceutical Sciences & Research, Pune 411 018, India. 2. Department of Pharmacology, Sinhgad Technical Education Society, Sinhgad Institute of Pharmaceutical Sciences, Lonavala, Pune 410 401, India. 3. Department of Pharmacology, Sinhgad Technical Education Society, Sinhgad Institute of Pharmaceutical Sciences, Lonavala, Pune 410 401, India. Electronic address: srnaik5@rediffmail.com.
Abstract
AIMS: The neuroprotective activities of silymarin, piracetam and protocatechuic acid ethyl ester (PCA) on cerebral global ischemic/reperfusion were evaluated in a rat model. MAIN METHODS: A midline ventral incision was made in the throat region. The right and left common carotid arteries were located and a bilateral common carotid artery occlusion (BCCAO) was performed for 30min using atraumatic clamps followed by a 24h period of reperfusion. Neurological/behavioral functions (cognitive and motor), endogenous defense systems (lipid peroxidation, glutathione, catalase, and superoxide dismutase), reduced water content and infarct size and histopathological alterations were then studied. KEY FINDINGS: Silymarin and PCA treatments significantly improved cognitive, motor and endogenous defense functions, histopathological alterations, and, reduced both water content and infarct size compared to the vehicle-treated ischemic control group. Piracetam treatment improved neurological and histopathological alterations, reduced water content and infarct size, but failed to restore/prevent the impaired endogenous defense functions significantly. SIGNIFICANCE: Silymarin showed better neuroprotection than piracetam and PCA in experimentally induced global ischemic/reperfusion and was able to facilitate mnemonic performance.
AIMS: The neuroprotective activities of silymarin, piracetam and protocatechuic acid ethyl ester (PCA) on cerebral global ischemic/reperfusion were evaluated in a rat model. MAIN METHODS: A midline ventral incision was made in the throat region. The right and left common carotid arteries were located and a bilateral common carotid artery occlusion (BCCAO) was performed for 30min using atraumatic clamps followed by a 24h period of reperfusion. Neurological/behavioral functions (cognitive and motor), endogenous defense systems (lipid peroxidation, glutathione, catalase, and superoxide dismutase), reduced water content and infarct size and histopathological alterations were then studied. KEY FINDINGS:Silymarin and PCA treatments significantly improved cognitive, motor and endogenous defense functions, histopathological alterations, and, reduced both water content and infarct size compared to the vehicle-treated ischemic control group. Piracetam treatment improved neurological and histopathological alterations, reduced water content and infarct size, but failed to restore/prevent the impaired endogenous defense functions significantly. SIGNIFICANCE: Silymarin showed better neuroprotection than piracetam and PCA in experimentally induced global ischemic/reperfusion and was able to facilitate mnemonic performance.
Authors: Vishnu N Thakare; Rajesh R Patil; Anupama A Suralkar; Valmik D Dhakane; Bhoomika M Patel Journal: Metab Brain Dis Date: 2019-03-08 Impact factor: 3.584
Authors: Song Hee Lee; Bo Young Choi; A Ra Kho; Jeong Hyun Jeong; Dae Ki Hong; Sang Hwon Lee; Sang Yup Lee; Min Woo Lee; Hong Ki Song; Hui Chul Choi; Sang Won Suh Journal: Int J Mol Sci Date: 2018-01-08 Impact factor: 5.923
Authors: A Ra Kho; Bo Young Choi; Song Hee Lee; Dae Ki Hong; Sang Hwon Lee; Jeong Hyun Jeong; Kyoung-Ha Park; Hong Ki Song; Hui Chul Choi; Sang Won Suh Journal: Int J Mol Sci Date: 2018-05-09 Impact factor: 5.923